Functional characterization of the H-2DP gene product

A lambda clone containing the Dp gene was used to transform L cells. The Dp product expressed was identified by two-dimensional gel electrophoresis and flow cytometry. The Dp product expressed by the L cells was recognized by Dp-specific but not Kp-specific killer T cells. This killing was inhibited...

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Bibliographic Details
Published in:Immunogenetics (New York) 1984, Vol.19 (3), p.205-216
Main Authors: MCLAUGHLIN-TAYLOR, E, WOODWARD, J. G, MACCHI, M. J, MCMILLAN, M, FRELINGER, J. A
Format: Article
Language:English
Subjects:
Animals
Bacteriophage lambda - genetics
Biological and medical sciences
Cloning, Molecular
Cytotoxicity, Immunologic
Electrophoresis, Polyacrylamide Gel
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Genetics of the immune response
H-2 Antigens - genetics
Histocompatibility Antigen H-2D
Immunobiology
Killer Cells, Natural - immunology
L Cells (Cell Line) - immunology
Lymphocytic choriomeningitis virus - immunology
Major Histocompatibility Complex
Mice
Mice, Inbred Strains
Nucleic Acid Hybridization
Transfection
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Summary:A lambda clone containing the Dp gene was used to transform L cells. The Dp product expressed was identified by two-dimensional gel electrophoresis and flow cytometry. The Dp product expressed by the L cells was recognized by Dp-specific but not Kp-specific killer T cells. This killing was inhibited by monoclonal antibodies specific for Dp but not Kp or Kk antigens. Similarly, lymphocytic choriomeningitis virus (LCMV) killer T cells from B10.P mice were able to kill LCMV-infected L12a cells, but not LCMV-infected Ltk+. Again only Dp monoclonal antibodies could inhibit this killing.
ISSN:0093-7711
1432-1211
DOI:10.1007/BF00364764