Cloning and identification of the H-2DP gene

We have cloned six different class I genes from a B10.P sperm library. After cotransfection with the herpes simplex tk gene, one L-cell line was found to react with six H-2Dp-specific monoclonal antibodies. The cell line L12a did not react with Kp-specific monoclonal antibodies. This identification...

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Bibliographic Details
Published in:Immunogenetics (New York) 1984, Vol.19 (3), p.195-204
Main Authors: MACCHI, M. J, WOODWARD, J. G, MCLAUGHLIN-TAYLOR, E, GRIFFIN, J, HOOD, L, FRELINGER, J. A
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Base Sequence
Biological and medical sciences
Cloning, Molecular
DNA Restriction Enzymes
Fundamental and applied biological sciences. Psychology
Genes. Genome
H-2 Antigens - genetics
Histocompatibility Antigen H-2D
L Cells (Cell Line) - immunology
Major Histocompatibility Complex
Male
Mice
Molecular and cellular biology
Molecular genetics
Simplexvirus - enzymology
Spermatozoa - immunology
Thymidine Kinase - genetics
Transfection
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Summary:We have cloned six different class I genes from a B10.P sperm library. After cotransfection with the herpes simplex tk gene, one L-cell line was found to react with six H-2Dp-specific monoclonal antibodies. The cell line L12a did not react with Kp-specific monoclonal antibodies. This identification was confirmed by mapping a 2.5 kb Bam H1 restriction fragment present in the lambda 12a DNA clone to the D-TL region of H-2p. Only a single 8.8 kb Bam H1 fragment can be assigned to Kp by restriction fragment length polymorphism, while many others map to the D-TL interval. A restriction map of lambda 12a is presented.
ISSN:0093-7711
1432-1211
DOI:10.1007/BF00364763