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Cloning and identification of the H-2DP gene
We have cloned six different class I genes from a B10.P sperm library. After cotransfection with the herpes simplex tk gene, one L-cell line was found to react with six H-2Dp-specific monoclonal antibodies. The cell line L12a did not react with Kp-specific monoclonal antibodies. This identification...
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| Published in: | Immunogenetics (New York) 1984, Vol.19 (3), p.195-204 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| container_end_page | 204 |
| container_issue | 3 |
| container_start_page | 195 |
| container_title | Immunogenetics (New York) |
| container_volume | 19 |
| creator | MACCHI, M. J WOODWARD, J. G MCLAUGHLIN-TAYLOR, E GRIFFIN, J HOOD, L FRELINGER, J. A |
| description | We have cloned six different class I genes from a B10.P sperm library. After cotransfection with the herpes simplex tk gene, one L-cell line was found to react with six H-2Dp-specific monoclonal antibodies. The cell line L12a did not react with Kp-specific monoclonal antibodies. This identification was confirmed by mapping a 2.5 kb Bam H1 restriction fragment present in the lambda 12a DNA clone to the D-TL region of H-2p. Only a single 8.8 kb Bam H1 fragment can be assigned to Kp by restriction fragment length polymorphism, while many others map to the D-TL interval. A restriction map of lambda 12a is presented. |
| doi_str_mv | 10.1007/BF00364763 |
| format | article |
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J ; WOODWARD, J. G ; MCLAUGHLIN-TAYLOR, E ; GRIFFIN, J ; HOOD, L ; FRELINGER, J. A</creator><creatorcontrib>MACCHI, M. J ; WOODWARD, J. G ; MCLAUGHLIN-TAYLOR, E ; GRIFFIN, J ; HOOD, L ; FRELINGER, J. A</creatorcontrib><description>We have cloned six different class I genes from a B10.P sperm library. After cotransfection with the herpes simplex tk gene, one L-cell line was found to react with six H-2Dp-specific monoclonal antibodies. The cell line L12a did not react with Kp-specific monoclonal antibodies. This identification was confirmed by mapping a 2.5 kb Bam H1 restriction fragment present in the lambda 12a DNA clone to the D-TL region of H-2p. Only a single 8.8 kb Bam H1 fragment can be assigned to Kp by restriction fragment length polymorphism, while many others map to the D-TL interval. A restriction map of lambda 12a is presented.</description><identifier>ISSN: 0093-7711</identifier><identifier>EISSN: 1432-1211</identifier><identifier>DOI: 10.1007/BF00364763</identifier><identifier>PMID: 6323309</identifier><identifier>CODEN: IMNGBK</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Animals ; Antibodies, Monoclonal ; Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; DNA Restriction Enzymes ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; H-2 Antigens - genetics ; Histocompatibility Antigen H-2D ; L Cells (Cell Line) - immunology ; Major Histocompatibility Complex ; Male ; Mice ; Molecular and cellular biology ; Molecular genetics ; Simplexvirus - enzymology ; Spermatozoa - immunology ; Thymidine Kinase - genetics ; Transfection</subject><ispartof>Immunogenetics (New York), 1984, Vol.19 (3), p.195-204</ispartof><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9649024$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6323309$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MACCHI, M. J</creatorcontrib><creatorcontrib>WOODWARD, J. G</creatorcontrib><creatorcontrib>MCLAUGHLIN-TAYLOR, E</creatorcontrib><creatorcontrib>GRIFFIN, J</creatorcontrib><creatorcontrib>HOOD, L</creatorcontrib><creatorcontrib>FRELINGER, J. A</creatorcontrib><title>Cloning and identification of the H-2DP gene</title><title>Immunogenetics (New York)</title><addtitle>Immunogenetics</addtitle><description>We have cloned six different class I genes from a B10.P sperm library. After cotransfection with the herpes simplex tk gene, one L-cell line was found to react with six H-2Dp-specific monoclonal antibodies. The cell line L12a did not react with Kp-specific monoclonal antibodies. This identification was confirmed by mapping a 2.5 kb Bam H1 restriction fragment present in the lambda 12a DNA clone to the D-TL region of H-2p. Only a single 8.8 kb Bam H1 fragment can be assigned to Kp by restriction fragment length polymorphism, while many others map to the D-TL interval. A restriction map of lambda 12a is presented.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>DNA Restriction Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>H-2 Antigens - genetics</subject><subject>Histocompatibility Antigen H-2D</subject><subject>L Cells (Cell Line) - immunology</subject><subject>Major Histocompatibility Complex</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Simplexvirus - enzymology</subject><subject>Spermatozoa - immunology</subject><subject>Thymidine Kinase - genetics</subject><subject>Transfection</subject><issn>0093-7711</issn><issn>1432-1211</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><recordid>eNo9jztPwzAURi0EKqWwsCNlQEwErn0dP0ZaKEWqBAPMkeNHMUqdEqcD_55KREzfcI6O9BFySeGOAsj7-RIABZcCj8iUcmQlZZQekymAxlJKSk_JWc5fALTSTEzIRCBDBD0lt4u2SzFtCpNcEZ1PQwzRmiF2qehCMXz6YlWyx7di45M_JyfBtNlfjDsjH8un98WqXL8-vywe1uWOCjmUXgSvVGWd8Mxx5YIG3tgGGCqlnLUYqkY7yxpmlGYObaDOQuDS24ajEjgjN3_dXd99730e6m3M1retSb7b51qBVrxCeRCvRnHfbL2rd33cmv6nHu8d-PXITbamDb1JNuZ_TQuugXH8BV52W7Y</recordid><startdate>1984</startdate><enddate>1984</enddate><creator>MACCHI, M. 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Genome</topic><topic>H-2 Antigens - genetics</topic><topic>Histocompatibility Antigen H-2D</topic><topic>L Cells (Cell Line) - immunology</topic><topic>Major Histocompatibility Complex</topic><topic>Male</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Simplexvirus - enzymology</topic><topic>Spermatozoa - immunology</topic><topic>Thymidine Kinase - genetics</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MACCHI, M. J</creatorcontrib><creatorcontrib>WOODWARD, J. G</creatorcontrib><creatorcontrib>MCLAUGHLIN-TAYLOR, E</creatorcontrib><creatorcontrib>GRIFFIN, J</creatorcontrib><creatorcontrib>HOOD, L</creatorcontrib><creatorcontrib>FRELINGER, J. 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A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and identification of the H-2DP gene</atitle><jtitle>Immunogenetics (New York)</jtitle><addtitle>Immunogenetics</addtitle><date>1984</date><risdate>1984</risdate><volume>19</volume><issue>3</issue><spage>195</spage><epage>204</epage><pages>195-204</pages><issn>0093-7711</issn><eissn>1432-1211</eissn><coden>IMNGBK</coden><abstract>We have cloned six different class I genes from a B10.P sperm library. After cotransfection with the herpes simplex tk gene, one L-cell line was found to react with six H-2Dp-specific monoclonal antibodies. The cell line L12a did not react with Kp-specific monoclonal antibodies. This identification was confirmed by mapping a 2.5 kb Bam H1 restriction fragment present in the lambda 12a DNA clone to the D-TL region of H-2p. Only a single 8.8 kb Bam H1 fragment can be assigned to Kp by restriction fragment length polymorphism, while many others map to the D-TL interval. A restriction map of lambda 12a is presented.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>6323309</pmid><doi>10.1007/BF00364763</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0093-7711 |
| ispartof | Immunogenetics (New York), 1984, Vol.19 (3), p.195-204 |
| issn | 0093-7711 1432-1211 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80984537 |
| source | SpringerLink_过刊（NSTL购买） |
| subjects | Animals Antibodies, Monoclonal Base Sequence Biological and medical sciences Cloning, Molecular DNA Restriction Enzymes Fundamental and applied biological sciences. Psychology Genes. Genome H-2 Antigens - genetics Histocompatibility Antigen H-2D L Cells (Cell Line) - immunology Major Histocompatibility Complex Male Mice Molecular and cellular biology Molecular genetics Simplexvirus - enzymology Spermatozoa - immunology Thymidine Kinase - genetics Transfection |
| title | Cloning and identification of the H-2DP gene |
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