Distinctive Characteristics of Crude Interferon from Virus-infected Guinea-pig Embryo Fibroblasts

• Published in: Journal of general virology 1984-04, Vol.65 (4), p.843-847
• Main Authors: Winship, Timothy R, Fong, C. K. Y, Hsiung, G. D
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cells, Cultured; Embryo, Mammalian; Fibroblasts; Fundamental and applied biological sciences. Psychology; Guinea Pigs; Hot Temperature; Hydrogen-Ion Concentration; Interferon Type I - antagonists & inhibitors; Interferon Type I - biosynthesis; L Cells (Cell Line); Mice; Microbiology; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Sodium Dodecyl Sulfate - pharmacology; Vesicular stomatitis Indiana virus - physiology; Virology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-65-4-843

* Virology Laboratory, Veterans Administration Medical Center, West Haven, Connecticut 06516 and Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06510, U.S.A. Crude interferon preparations from primary guinea-pig embryo cells infected with vesicular stomatitis virus strain T1026R1 were shown to be more sensitive to heat (37 °C), pH 2.0, and SDS than crude mouse interferon. Since the proportion of antiviral activity lost after each treatment was nearly the same, the existence of a single fraction of antiviral activity sensitive to all three treatments was suggested. Support for this possibility was given by the finding that subjecting this guinea-pig interferon to any one of the treatments rendered it insensitive to the effects of the other two. Keywords: IFN, guinea-pig, acid-labile Present address: Daryl Laboratories, 2220 Martin Ave., Santa Clara, Californai 95050, U.S.A. Received 13 July 1983; accepted 13 January 1984.