Application of solid-phase immunofluorometric assay to the selection of monoclonal antibody specific for the adenovirus group-reactive hexon antigen

• Published in: Archives of virology 1984-01, Vol.80 (1), p.1-10
• Main Authors: HIERHOLZER, J. C, PHILLIPS, D. J, HUMPHREY, D. D, COOMBS, R. A, REIMER, C. B
• Format: Article
• Language: English
• Subjects: adenovirus; Adenoviruses, Human - immunology; Animals; Antibodies, Monoclonal - analysis; Antibodies, Monoclonal - immunology; Antibodies, Viral - analysis; Ascitic Fluid - immunology; Biological and medical sciences; Capsid - immunology; Capsid Proteins; Complement Fixation Tests; Counterimmunoelectrophoresis; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Hemagglutination Inhibition Tests; Hybridomas; Mice; Mice, Inbred BALB C; Microbiology; Neutralization Tests; Spectrometry, Fluorescence; Techniques used in virology; Virology
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/BF01315289

An immunofluorometric assay (IFMA) has been evaluated as a screening assay to detect monoclonal antibodies to the group-specific antigen of the adenovirus hexon component. The antibodies were produced as mouse ascitic fluids from hybridoma cells generated from Balb/C mice immunized with purified adenovirus type 2 hexon component and crude adenovirus type 3 culture supernatants. The purified IgG fractions from all monoclonal ascitic fluids tested were identified as the IgG1 K mouse isotype. Antibody titers ranged from 102,400 to 204,800 by the IFMA, from 200 to 12,800 by indirect FA, and were generally nonreactive in counterelectrophoresis, complement fixation, hemagglutination-inhibition, serum neutralization, and immune electron microscopy titrations. The IFMA is a reliable method for quantitating low levels of specific antibody in large numbers of test samples, and is therefore ideal as a screening assay for monoclonal antibody in tissue culture fluids and in mouse ascitic fluids.