Chromatin regions, released by endogenous nucleases, are enriched in immunogenic tissue-specific proteins

Localization of immunogenic tissue-specific proteins in chromatin regions, hypersensitive to endogenous nucleases, has been studied using rabbit antibodies against rat thymus chromatin. It is shown that the first 1-2,5% of the chromatin (calculating on DNA), released by Mg2+-, Mn2+- and Ca2+/Mg2+-de...

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Bibliographic Details
Published in:Molecular biology reports 1984, Vol.9 (4), p.263-267
Main Authors: ERMEKOVA, V. M, MELKONYAN, O. S, ZOTOVA, R. N, NAZAROVA, L. F, UMANSKY, S. R
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Chromatin - immunology
Chromatin - ultrastructure
Chromatin. Chromosome
Deoxyribonucleases - metabolism
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Molecular genetics
Rabbits
Rats
Solubility
Thymus Gland - immunology
Tissue Distribution
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Summary:Localization of immunogenic tissue-specific proteins in chromatin regions, hypersensitive to endogenous nucleases, has been studied using rabbit antibodies against rat thymus chromatin. It is shown that the first 1-2,5% of the chromatin (calculating on DNA), released by Mg2+-, Mn2+- and Ca2+/Mg2+-dependent nuclear endonucleases are drastically enriched in tissue-specific antigenic determinants. The released chromatin fractions are found to contain a heterogeneous set of nonhistone proteins and are deficient in histones. The cleavage of nuclear DNA by endogenous acidic nuclease, independent on bivalent ions, resulted in a significantly less enrichment of the released fractions with immunogenic proteins.
ISSN:0301-4851
1573-4978
DOI:10.1007/BF00775359