Time dependent conversion of creatine kinase MM isoforms in man

Subforms of creatine kinase MM isoenzyme (isoforms, pI: MMA=7.95, MMB=7.76, MMC=7.54) in human myocardium and serum were quantified by chromatofocusing. Creatine kinase MMA was a dominant isoform (>94% of MM) in normal (n=3) and in both infarcted and non-infarcted myocardium (n=2). To investigate...

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Bibliographic Details
Published in:Cardiovascular research 1987-06, Vol.21 (6), p.433-438
Main Authors: KOJIMA, TAKAHIKO, HASHIMOTO, HIDEKAZU, TSUKAMOTO, HIDETO, ITO, TAKAYUKI, OGAWA, KOUICHI, SATAKE, TATSUO
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cardiology. Vascular system
chromatofocusing
Coronary heart disease
creatine kinase
Creatine Kinase - blood
Creatine Kinase - metabolism
disappearance rate
Heart
Humans
isoelectric point
Isoenzymes
isoform
Isomerism
Medical sciences
myocardial infarction
Myocardial Infarction - enzymology
Myocardium - enzymology
Time Factors
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Summary:Subforms of creatine kinase MM isoenzyme (isoforms, pI: MMA=7.95, MMB=7.76, MMC=7.54) in human myocardium and serum were quantified by chromatofocusing. Creatine kinase MMA was a dominant isoform (>94% of MM) in normal (n=3) and in both infarcted and non-infarcted myocardium (n=2). To investigate isoform conversion in vitro partially purified MMA was incubated with human plasma at 37°C for 24 h (n=5). Creatine kinase MMB (0%, 47%, 44% of MM at 0, 12, 24 h respectively) and MMC (0%, 22%, 42%) sequentially appeared in incubation media whereas MMA (100%, 31%, 14%) disappeared rapidly with a mean disappearance rate of -0.00169(0.00021)(SD) min−1. Individual differences in conversion velocity were small (SD
ISSN:0008-6363
1755-3245
DOI:10.1093/cvr/21.6.433