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Demonstration of 1,25(OH)2 vitamin D3 receptors and actions in vascular smooth muscle cells in vitro
Binding of [3H] 1,25(OH)2D3 and effects of 1,25(OH)2D3 on cell ultrastructure were evaluated in vascular smooth muscle cells (VSMC) primary cultures (aortic media). Specific reversible binding of [3H] 1,25(OH)2D3 by a 3.5 S macromolecule with DNA binding, KD 6.2 X 10(-10) M and Nmax 16 fmol/mg prote...
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| Published in: | Calcified tissue international 1987-08, Vol.41 (2), p.112-114 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| container_end_page | 114 |
| container_issue | 2 |
| container_start_page | 112 |
| container_title | Calcified tissue international |
| container_volume | 41 |
| creator | MERKE, J HOFMANN, W GOLDSCHMIDT, D RITZ, E |
| description | Binding of [3H] 1,25(OH)2D3 and effects of 1,25(OH)2D3 on cell ultrastructure were evaluated in vascular smooth muscle cells (VSMC) primary cultures (aortic media). Specific reversible binding of [3H] 1,25(OH)2D3 by a 3.5 S macromolecule with DNA binding, KD 6.2 X 10(-10) M and Nmax 16 fmol/mg protein was demonstrated. Incubation of VSMC with 10(-8) M 1,25(OH)2D3, but not 25(OH)D3, in the presence of 10% FCS for up to three weeks caused rapid reversible appearance in the cytoplasm of membrane-bounded electron-dense lysosomal particles which on electronspectroscopic imaging contained Ca and Pi. VSMC are targets for vitamin D. |
| doi_str_mv | 10.1007/BF02555253 |
| format | article |
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Specific reversible binding of [3H] 1,25(OH)2D3 by a 3.5 S macromolecule with DNA binding, KD 6.2 X 10(-10) M and Nmax 16 fmol/mg protein was demonstrated. Incubation of VSMC with 10(-8) M 1,25(OH)2D3, but not 25(OH)D3, in the presence of 10% FCS for up to three weeks caused rapid reversible appearance in the cytoplasm of membrane-bounded electron-dense lysosomal particles which on electronspectroscopic imaging contained Ca and Pi. VSMC are targets for vitamin D.</description><identifier>ISSN: 0171-967X</identifier><identifier>EISSN: 1432-0827</identifier><identifier>DOI: 10.1007/BF02555253</identifier><identifier>PMID: 2820558</identifier><identifier>CODEN: CTINDZ</identifier><language>eng</language><publisher>New York, NY: Springer-Verlag</publisher><subject>Animals ; Biological and medical sciences ; Blood vessels and receptors ; Calcitriol - metabolism ; Calcitriol - pharmacology ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Male ; Muscle, Smooth, Vascular - drug effects ; Muscle, Smooth, Vascular - metabolism ; Muscle, Smooth, Vascular - ultrastructure ; Rabbits ; Rats ; Rats, Inbred Strains ; Receptors, Calcitriol ; Receptors, Steroid - metabolism ; Vertebrates: cardiovascular system</subject><ispartof>Calcified tissue international, 1987-08, Vol.41 (2), p.112-114</ispartof><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7429993$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2820558$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MERKE, J</creatorcontrib><creatorcontrib>HOFMANN, W</creatorcontrib><creatorcontrib>GOLDSCHMIDT, D</creatorcontrib><creatorcontrib>RITZ, E</creatorcontrib><title>Demonstration of 1,25(OH)2 vitamin D3 receptors and actions in vascular smooth muscle cells in vitro</title><title>Calcified tissue international</title><addtitle>Calcif Tissue Int</addtitle><description>Binding of [3H] 1,25(OH)2D3 and effects of 1,25(OH)2D3 on cell ultrastructure were evaluated in vascular smooth muscle cells (VSMC) primary cultures (aortic media). Specific reversible binding of [3H] 1,25(OH)2D3 by a 3.5 S macromolecule with DNA binding, KD 6.2 X 10(-10) M and Nmax 16 fmol/mg protein was demonstrated. Incubation of VSMC with 10(-8) M 1,25(OH)2D3, but not 25(OH)D3, in the presence of 10% FCS for up to three weeks caused rapid reversible appearance in the cytoplasm of membrane-bounded electron-dense lysosomal particles which on electronspectroscopic imaging contained Ca and Pi. VSMC are targets for vitamin D.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood vessels and receptors</subject><subject>Calcitriol - metabolism</subject><subject>Calcitriol - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Muscle, Smooth, Vascular - ultrastructure</subject><subject>Rabbits</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Receptors, Calcitriol</subject><subject>Receptors, Steroid - metabolism</subject><subject>Vertebrates: cardiovascular system</subject><issn>0171-967X</issn><issn>1432-0827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNo9kMFLwzAUh4Moc04v3oUcRBSsJi9Nkx51c04Y7KLgrWRJipW2qUk68L-3Y0V48A7fx-P9fghdUvJACRGPz0sCnHPg7AhNacogIRLEMZoSKmiSZ-LzFJ2F8E0ITbMsm6AJSCCcyykyC9u4NkSvYuVa7EpM74HfblZ3gHdVVE3V4gXD3mrbRecDVq3BSu_lgAe2U0H3tfI4NM7FL9z0QdcWa1vXB15F787RSanqYC_GPUMfy5f3-SpZb17f5k_rpKOcxERrkEMcoqnaQmq00ARIKYSUPAeRlykVzA6zNZCVViluZJab0hijiAXQbIZuDnc77356G2LRVGH_imqt60MhKaEyY2IQr0ax3zbWFJ2vGuV_i7GWgV-PfIin6tKrVlfhXxMp5HnO2B-R02_E</recordid><startdate>198708</startdate><enddate>198708</enddate><creator>MERKE, J</creator><creator>HOFMANN, W</creator><creator>GOLDSCHMIDT, D</creator><creator>RITZ, E</creator><general>Springer-Verlag</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>198708</creationdate><title>Demonstration of 1,25(OH)2 vitamin D3 receptors and actions in vascular smooth muscle cells in vitro</title><author>MERKE, J ; HOFMANN, W ; GOLDSCHMIDT, D ; RITZ, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p150t-cc280070c1ab24dc7c020f778859279f4173e73ebd26feaa5d869dfddda0e22c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood vessels and receptors</topic><topic>Calcitriol - metabolism</topic><topic>Calcitriol - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Muscle, Smooth, Vascular - ultrastructure</topic><topic>Rabbits</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Receptors, Calcitriol</topic><topic>Receptors, Steroid - metabolism</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MERKE, J</creatorcontrib><creatorcontrib>HOFMANN, W</creatorcontrib><creatorcontrib>GOLDSCHMIDT, D</creatorcontrib><creatorcontrib>RITZ, E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Calcified tissue international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MERKE, J</au><au>HOFMANN, W</au><au>GOLDSCHMIDT, D</au><au>RITZ, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Demonstration of 1,25(OH)2 vitamin D3 receptors and actions in vascular smooth muscle cells in vitro</atitle><jtitle>Calcified tissue international</jtitle><addtitle>Calcif Tissue Int</addtitle><date>1987-08</date><risdate>1987</risdate><volume>41</volume><issue>2</issue><spage>112</spage><epage>114</epage><pages>112-114</pages><issn>0171-967X</issn><eissn>1432-0827</eissn><coden>CTINDZ</coden><abstract>Binding of [3H] 1,25(OH)2D3 and effects of 1,25(OH)2D3 on cell ultrastructure were evaluated in vascular smooth muscle cells (VSMC) primary cultures (aortic media). Specific reversible binding of [3H] 1,25(OH)2D3 by a 3.5 S macromolecule with DNA binding, KD 6.2 X 10(-10) M and Nmax 16 fmol/mg protein was demonstrated. Incubation of VSMC with 10(-8) M 1,25(OH)2D3, but not 25(OH)D3, in the presence of 10% FCS for up to three weeks caused rapid reversible appearance in the cytoplasm of membrane-bounded electron-dense lysosomal particles which on electronspectroscopic imaging contained Ca and Pi. VSMC are targets for vitamin D.</abstract><cop>New York, NY</cop><pub>Springer-Verlag</pub><pmid>2820558</pmid><doi>10.1007/BF02555253</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0171-967X |
| ispartof | Calcified tissue international, 1987-08, Vol.41 (2), p.112-114 |
| issn | 0171-967X 1432-0827 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81018637 |
| source | Springer Online Journal Archives (Through 1996) |
| subjects | Animals Biological and medical sciences Blood vessels and receptors Calcitriol - metabolism Calcitriol - pharmacology Fundamental and applied biological sciences. Psychology In Vitro Techniques Male Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - metabolism Muscle, Smooth, Vascular - ultrastructure Rabbits Rats Rats, Inbred Strains Receptors, Calcitriol Receptors, Steroid - metabolism Vertebrates: cardiovascular system |
| title | Demonstration of 1,25(OH)2 vitamin D3 receptors and actions in vascular smooth muscle cells in vitro |
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