Vimentin filaments in spreading, randomly locomoting, and f-met-leu-phe-treated neutrophils

Human neutrophils contain intermediate filaments of the vimentin type. A cytoskeletal preparation, produced by high-salt and Triton X-100 extraction of human neutrophils, reveals a major band at 57000 Mr that comigrates with 3T3 cell vimentin on one-dimensional gels. Two-dimensional gel electrophore...

Full description

Saved in:
Bibliographic Details
Published in:Cell and tissue research 1984-01, Vol.235 (3), p.575-581
Main Authors: Parysek, L M, Eckert, B S
Format: Article
Language:English
Subjects:
Autoradiography
Electrophoresis
Fluorescent Antibody Technique
Humans
Immunosorbent Techniques
In Vitro Techniques
Intermediate Filament Proteins - analysis
Microscopy, Electron
N-Formylmethionine Leucyl-Phenylalanine - pharmacology
Neutrophils - analysis
Neutrophils - drug effects
Neutrophils - ultrastructure
Vimentin
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human neutrophils contain intermediate filaments of the vimentin type. A cytoskeletal preparation, produced by high-salt and Triton X-100 extraction of human neutrophils, reveals a major band at 57000 Mr that comigrates with 3T3 cell vimentin on one-dimensional gels. Two-dimensional gel electrophoresis of whole neutrophils illustrates the presence of vimentin but not desmin- or keratin-filament subunits. The presence of vimentin in neutrophils is also shown by its specific staining with avian vimentin antiserum by two-dimensional gel immunoautoradiography. Indirect immunofluorescence studies show that vimentin antiserum labels an area on one side of the nucleus in spreading neutrophils. This bright area appears as a loose knot of vimentin filaments; a few filaments may radiate from the knot. In contrast to spreading neutrophils, those undergoing random locomotion contain a fine network of filaments that are located in the cytoplasm between the nucleus and the trailing end of the cell. Similarly, in chemoattractant-treated neutrophils, vimentin filaments are bundled in the uropod. Transmission electron microscopy of human neutrophil monolayers confirms the intracellular distribution of intermediate filaments as shown by immunofluorescence in spreading and randomly locomoting cells.
ISSN:0302-766X
1432-0878
DOI:10.1007/BF00226955