Estimation of aneuploidy levels in human spermatozoa using chromosome specific probes and in situ hybridisation

The paper describes an attempt to estimate the frequency of aneuploid human spermatozoa with disomic Y chromosome and disomic chromosome 1 complements, using chromosome specific probes and in situ hybridisation. This approach was used as an alternative to the differential staining techniques that ha...

Full description

Saved in:
Bibliographic Details
Published in:Human genetics 1984-04, Vol.66 (2-3), p.234-238
Main Authors: JOSEPH, A. M, GOSDEN, J. R, CHANDLEY, A. C
Format: Article
Language:English
Subjects:
Aged
Aneuploidy
Autoradiography
Biological and medical sciences
Chromosome aberrations
DNA - genetics
Female
Humans
Lymphocytes - ultrastructure
Male
Medical genetics
Medical sciences
Middle Aged
Nucleic Acid Hybridization
RNA - genetics
Spermatozoa - ultrastructure
Testis - ultrastructure
X Chromosome
Y Chromosome
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The paper describes an attempt to estimate the frequency of aneuploid human spermatozoa with disomic Y chromosome and disomic chromosome 1 complements, using chromosome specific probes and in situ hybridisation. This approach was used as an alternative to the differential staining techniques that have been applied to spermatozoa in previous studies aimed at estimating levels of aneuploidy for chromosome 1 and the Y chromosome. A frequency of 1.8 per 1000 YY-bearing spermatozoa and 3.5 per 1000 disomy 1 spermatozoa was found, both figures being in excess of those found by sperm genome karyotyping. The technical limitations of the method are discussed.
ISSN:0340-6717
1432-1203
DOI:10.1007/BF00286608