Karyotyping and identification of human chromosome polymorphisms by single fluorochrome flow cytometry

Frequency distributions of fluorescence intensity of ethidium bromide stained human chromosomes from nine phenotypically normal males are cross correlated and autocorrelated following repeated flow cytometric measurements. It is shown that each individual donor produces a fluorescence profile which...

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Bibliographic Details
Published in:Human genetics 1984-01, Vol.66 (2-3), p.143-146
Main Authors: GREEN, D. K, FANTES, J. A, BUCKTON, K. E, ELDER, J. K, MALLOY, P, CAROTHERS, A, EVANS, H. J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromosome Banding
Chromosomes, Human
Chromosomes, Human, 13-15
Cytogenetics
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Human
Humans
Karyotyping - methods
Lymphocytes - ultrastructure
Male
Polymorphism, Genetic
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Summary:Frequency distributions of fluorescence intensity of ethidium bromide stained human chromosomes from nine phenotypically normal males are cross correlated and autocorrelated following repeated flow cytometric measurements. It is shown that each individual donor produces a fluorescence profile which is both visually and numerically different from those of other individuals in the set. The wide variety of chromosome heteromorphisms which occur to varying degrees for chromosomes 1, 9, 13, 14, 15, 16, 21, 22 and Y give rise to the uniqueness of a given fluorescence profile. Estimates of chromosome heteromorphisms for each individual in the set were made and then compared with parallel results obtained from inspection of Q-banded and C-banded conventional metaphase preparations. Fluorescence profiles identifiable with each individual were also obtained for Hoechst 33258 stained chromosomes.
ISSN:0340-6717
1432-1203
DOI:10.1007/BF00286588