Molecular and genetic studies on the euchromatin-heterochromatin transition region of the X chromosome of Drosophila melanogaster. 1. A cloned entry point near to the uncoordinated (unc) locus

A recombinant Charon 4 bacteriophage has been isolated on the basis of RNAs which are enriched in the head of the adult Drosophila melanogaster and hence are likely to be of neural origin. The cloned insert maps to the near vicinity of the uncoordinated locus in polytene chromosome band 19E8. This b...

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Bibliographic Details
Published in:Chromosoma 1984-01, Vol.89 (3), p.218-227
Main Authors: Miklos, G.L.G, Healy, M.J, Pain, P, Howells, A.J, Russell, R.J
Format: Article
Language:English
Subjects:
animal breeding
animal genetics
Animals
arthropods
Bacteriophage lambda
Chromatin - genetics
Chromosome Mapping
Cloning, Molecular
DNA - genetics
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophilidae
entomology
Euchromatin
Female
Heterochromatin - genetics
Nucleic Acid Hybridization
Repetitive Sequences, Nucleic Acid
RNA - genetics
X Chromosome - ultrastructure
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Summary:A recombinant Charon 4 bacteriophage has been isolated on the basis of RNAs which are enriched in the head of the adult Drosophila melanogaster and hence are likely to be of neural origin. The cloned insert maps to the near vicinity of the uncoordinated locus in polytene chromosome band 19E8. This band is within the transition zone between the euchromatic and heterochromatic regions of the X chromosome, a region which has been well characterized cytogenetically. The insert contains both repetitious and low copy number sequences, some of which vary extensively in both frequency and restriction fragment size between different laboratory strains. One particular family of moderately repeated sequences occurs predominantly in divisions 19 and 20 of the X chromosome and perhaps the distally located X heterochromatin. The molecular landscape surrounding the initial entry point contains many repeated sequences and is thus unlike those observed in most published chromosomal walks. The possible significance of the presence of repeated families in the distinct properties of this region are discussed.
ISSN:0009-5915
1432-0886
DOI:10.1007/BF00295003