Immunoreactivity of Cytomegalovirus-Induced Fc Receptors

The present studies were undertaken to characterize the affinity of CMV-induced Fc receptors for each of the subclasses of human IgG and to define the specific region of the IgG Fc fragment interacting with such receptors. To do this, we infected confluent human embryonic lung (HEL) cell monolayers...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1987, Vol.31(5), pp.427-434
Main Authors: Mackowiak, Philip A., Marling-Cason, Margaret
Format: Article
Language:English
Subjects:
Bacterial Adhesion
Bacteriology
Biological and medical sciences
Cell Line
Cytomegalovirus - immunology
Escherichia coli
Escherichia coli - metabolism
Fibroblasts
Fundamental and applied biological sciences. Psychology
Human cytomegalovirus
Humans
Immunoglobulin Fc Fragments - immunology
Immunoglobulin G - immunology
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Receptors, Fc - immunology
Staphylococcal Protein A - metabolism
Temperature
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Summary:The present studies were undertaken to characterize the affinity of CMV-induced Fc receptors for each of the subclasses of human IgG and to define the specific region of the IgG Fc fragment interacting with such receptors. To do this, we infected confluent human embryonic lung (HEL) cell monolayers with CMV (strain AD169) and then used a double radiolabel assay to measure adherence of antibody-coated E. coli 06 to such monolayers. Preincubating monolayers with each of the 4 subclasses of human IgG (but not IgA, IgM, or human or bovine albumin) abrogated the enhancing effect of CMV infection on adherence of antibody-coated E. coli 06 to HEL monolayers. Pepsin-derived, purified Fc fragments of human IgG had a similar abrogative effect. Preincubating these with staphylococcal protein A did not reduce their capacity to interfere with binding of antibody-coated E. coli to CMV-induced Fc receptors. These observations establish a broad range of immunoreactivity for CMV-induced Fc receptors, that encompasses all 4 subclasses of human IgG. They also provide indirect evidence that the reaction site of CMV-induced Fc receptors is in the CH2 domain of the Fc fragment.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1987.tb03105.x