Ion permeability of rabbit intestinal brush border membrane vesicles

The ion permeability of rabbit jejunal brush border membrane vesicles was studied by measuring unidirectional fluxes with radioactive tracers and bi-ionic diffusion potentials with the potential-sensitive fluorescent dye, diS-C3-(5). Tracer measurements provide estimates of the absolute magnitudes o...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of membrane biology 1984-01, Vol.78 (2), p.119-127
Main Authors: GUNTHER, R. D, SCHELL, R. E, WRIGHT, E. M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
brush border membranes
Cell Membrane Permeability
Cell physiology
Fundamental and applied biological sciences. Psychology
In Vitro Techniques
Ions
jejunum
Jejunum - metabolism
Jejunum - ultrastructure
Membrane and intracellular transports
Membrane Potentials
Microvilli - metabolism
Molecular and cellular biology
Potassium - metabolism
Rabbits
Sodium - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The ion permeability of rabbit jejunal brush border membrane vesicles was studied by measuring unidirectional fluxes with radioactive tracers and bi-ionic diffusion potentials with the potential-sensitive fluorescent dye, diS-C3-(5). Tracer measurements provide estimates of the absolute magnitudes of permeability coefficients, while fluorescence measurements provide estimates of relative and absolute ion permeabilities. The magnitudes of the permeability coefficients for Na+, K+, Rb+, and Br- were approximately 5 nanoliters/(mg protein X sec) or 10(-5) cm/sec as determined by radioactive tracer measurements. The apparent selectivity sequence, relative to Na+, as determined by bi-ionic potential measurements was: F-, isethionate, gluconate, choline (less than 0.1) less than Na+(1.0) less than Cl-(1.5) = NO-3(1.5) less than Br-(2.3) less than K+(2.4) less than Rb+(2.5) less than Cs+(2.6) less than Li+(3.9) less than NH+4(12) less than I-(40). The origin of this selectivity sequence and its relationship to the ion permeability of the brush border membrane in the intact epithelium are discussed.
ISSN:0022-2631
1432-1424
DOI:10.1007/BF01869199