Isolation of Renin-Rich Rat Kidney Cells

Enzymatic dispersion and density gradient (Percoll) sedimentation were used to isolate a population of renin-containing, granule-laden cells (density 1.067 g/ml) from rat kidney cortex. Using immunohistochemistry (light microscopy) and electron microscopy, we defined the presence and ability of thes...

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Bibliographic Details
Published in:Hypertension (Dallas, Tex. 1979) Tex. 1979), 1987-11, Vol.10 (5), p.488-496
Main Authors: JOHNS, DEARING W, CAREY, ROBERT M, GOMEZ, R ARIEL, LYNCH, KEVIN, INAGAMI, TADASHI, SAYE, JOANNE, GEARY, KATALJN, FARNSWORTH, DIANE E, PEACH, MICHAEL J
Format: Article
Language:English
Subjects:
Animals
Arterial hypertension. Arterial hypotension
Biological and medical sciences
Blood and lymphatic vessels
Cardiology. Vascular system
DNA - genetics
Experimental diseases
Hemolytic Plaque Technique
Immunoenzyme Techniques
Immunohistochemistry
Juxtaglomerular Apparatus - analysis
Juxtaglomerular Apparatus - cytology
Juxtaglomerular Apparatus - ultrastructure
Male
Medical sciences
Microscopy, Electron
Nucleic Acid Hybridization
Rats
Rats, Inbred WKY
Renin - analysis
Renin - genetics
RNA - analysis
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Summary:Enzymatic dispersion and density gradient (Percoll) sedimentation were used to isolate a population of renin-containing, granule-laden cells (density 1.067 g/ml) from rat kidney cortex. Using immunohistochemistry (light microscopy) and electron microscopy, we defined the presence and ability of these cells to store renin protein(s). A 1000 base pair rat renin complementary DNA was used to show that these cells express the renin gene. The reverse hemolytic plaque assay defined the functional properties of the renin-containing cell. The data are consistent with the postulated inverse relationship between calcium concentration and release of renin. Thus, we have isolated a population of functional rat kidney cells that synthesize, store, and release renin.
ISSN:0194-911X
1524-4563
DOI:10.1161/01.HYP.10.5.488