T Lymphocyte Repopulation and Differentiation After Bone Marrow Transplantation. Early Shifts in the Ratio Between T4+ and T8+ T Lymphocytes Correlate With the Occurrence of Acute Graft-Versus-Host Disease

Acute graft-versus-host disease (GVHD), a major complication of allogeneic bone marrow transplantation (BMT), is probably mediated by T lymphocytes present in the marrow graft. In this study, the repopulation of the peripheral blood with T4+ and T8+ T cells was investigated during the period precedi...

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Bibliographic Details
Published in:Blood 1984-06, Vol.63 (6), p.1416-1423
Main Authors: Gratama, Jan W., Naipal, Albert, Oljans, Paul, Zwaan, Ferry E., Verdonck, Leo F., de Witte, Theo, Vossen, Jaak M.J.J., Bolhuis, Reinder L.H., de Gast, Gijsbert C., Jansen, Jan
Format: Article
Language:English
Subjects:
Adolescent
Adult
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Biological and medical sciences
Bone Marrow Transplantation
Bone marrow, stem cells transplantation. Graft versus host reaction
Cell Survival
Female
Graft vs Host Disease - etiology
Humans
Leukocyte Count
Lymphocytes
Male
Medical sciences
T-Lymphocytes - classification
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
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Summary:Acute graft-versus-host disease (GVHD), a major complication of allogeneic bone marrow transplantation (BMT), is probably mediated by T lymphocytes present in the marrow graft. In this study, the repopulation of the peripheral blood with T4+ and T8+ T cells was investigated during the period preceding the occurrence of acute GVHD. Twenty-four allogeneic and 11 autologous BMT recipients were monitored from day 4 post-BMT onward by the use of monoclonal antibodies, indirect immunofluorescence, and flow cytometry. The recipients of allogeneic transplants received methotrexate as GVHD prophylaxis. Similar recovery patterns for T4+ and T8+ T cells were found following autologous and allogeneic BMT. However, lymphoid repopulation occurred at a clearly faster rate after autologous BMT. T4+ T cells were the first to reappear in the peripheral blood, followed by T8+ T cells 4-7 days later. The T8+ T cell reconstitution occurred at an even faster rate in patients who were to develop grade II-IV GVHD, as compared with those with grade ***0-I GVHD, thus leading to an earlier decrease in the T4/T8 ratio. Of 10 patients with a T4/T8 ratio 2.5 at that time, only 2 developed grade II—IV and 12 grade ***0-I GVHD (p < 0.001). In the 11 patients developing grade II-IV GVHD, the T4/T8 ratio decreased to values
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V63.6.1416.1416