Secretion of 5-hydroxytryptamine from electropermeabilised human platelets Effects of GTP and cyclic 3′, 5′-AMP

Enhancement by thrombin of Ca 2+ -dependent 5HT secretion in the absence of added GTP decreases as the time between electropermeabilisation and addition of thrombin is increased. No decrease occurs if thrombin is added with GTP. Observation of apparent GTP-independent receptor/phospholipase C coupli...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1987-10, Vol.223 (1), p.47-52
Main Authors: Knight, D.E., Scrutton, M.C.
Format: Article
Language:English
Subjects:
Blood Platelets - metabolism
Ca 2
Ca2
Calcium - physiology
Cell Membrane Permeability
cyclic AMP
Cyclic AMP - pharmacology
Electricity
GTP
Guanosine Triphosphate - pharmacology
Humans
In Vitro Techniques
man
Platelet
platelets
Secretion
serotonin
Serotonin - metabolism
Thrombin
Thrombin - pharmacology
Time Factors
Type C Phospholipases - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Enhancement by thrombin of Ca 2+ -dependent 5HT secretion in the absence of added GTP decreases as the time between electropermeabilisation and addition of thrombin is increased. No decrease occurs if thrombin is added with GTP. Observation of apparent GTP-independent receptor/phospholipase C coupling may result from the presence of bound GTP in the preparation. Enhancement by GTP of Ca 2+ -dependent 5HT secretion occurs with a significant lag indicating an agonist-independent effect. Cyclic 3′,5′-AMP inhibits enhancement by GTP of Ca 2+ -dependent 5HT secretion while having no effect on enhancement induced by GTPγS. Hence cyclic AMP may impair receptor/phospholipase C coupling by enhancing N p GTPase activity.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(87)80507-0