Binding of interleukin 2 to gangliosides

Exogenous gangliosides inhibit interleukin 2 (IL2)-dependent growth of a T cell line, AKIL-1.E8. IL2 activity is retained by columns of ganglioside covalently linked to poly(L-lysine)-agarose and is not eluted with ethylene glycol but is completely recovered by elution with 1% SDS. The ability of ga...

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Bibliographic Details
Published in:FEBS letters 1984-05, Vol.170 (2), p.391-395
Main Authors: Parker, Judy, Caldini, Gabriella, Krishnamurti, Chitra, Ahrens, Patricia B., Ankel, Helmut
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Animals
Biological and medical sciences
BSA, bovine serum albumin
Carbohydrates
Cattle
Cell Division - drug effects
Cell interactions
Cell Line
Ceramides - pharmacology
EG, ethylene glycol
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Ganglioside
gangliosides
Gangliosides - metabolism
Gangliosides - pharmacology
GD1a, NeuNAcα2-3Galβ1-3GalNAcβ1-4[NeuNAcα2-3]Galβ1-4Glc-Cer
GD1b, Galβ1-3GalNAcβ1-4[NeuNAcα2-8 NeuNAcα2-3]Galβ1-4Glc-Cer
globoside, GalNAcβ1-3Galα1-4Galβ1-4Glc-Cer
GM1, Galβ1-3GalNAcβ1-4[NeuNAcα2-3]-Galβ1-4Glc-Cer
GM2, GalNAcβ1-4[Neu-NAcα2-3]Galβ1-4Glc-Cer
GM3, NeuNAcα2-3Galβ1-4Glc-Cer
GT1b, NeuNAcα2-3Galβ1-3[NeuNAcα2-8 NeuNAcα2-3]GalNAcβ1-4Galβ1-4Glc-Cer
Hepes, N-hydroxyethylpiperazine-N-2-ethanesulfonic acid
Humans
IL2, interleukin 2
Immunobiology
Interleukin 2
Interleukin-2 - physiology
lymphocytes T
Lymphokine
Mice
PBS, phosphate buffer
Rats
Structure-Activity Relationship
T cell
T cell growth factor
T lymphocyte
T-Lymphocytes - cytology
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Summary:Exogenous gangliosides inhibit interleukin 2 (IL2)-dependent growth of a T cell line, AKIL-1.E8. IL2 activity is retained by columns of ganglioside covalently linked to poly(L-lysine)-agarose and is not eluted with ethylene glycol but is completely recovered by elution with 1% SDS. The ability of gangliosides to inhibit IL2 activity is directly related to the complexity of their carbohydrate portion, and related ceramide derivatives at similar concentrations do not inhibit IL2 activity. We conclude that IL2 bound to exogenous gangliosides is inactive and that the carbohydrate portion of the ganglioside is crucial to its interaction with IL2.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(84)81351-4