Functional groups on ‘Z’ DNA recognized by monoclonal antibodies

Both brominated poly[d(GC)] and poly[d(Gm 5C)] form stable left-handed Z-DNA structures at physiological ionic strengths. These two antigens were use to prepare monoclonal antibodies from immunized mice. The specificity of the antibodies was studied in detail with a solid-phase radioimmune assay as...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1984-03, Vol.168 (2), p.303-306
Main Authors: Lee, Jeremy S., Woodsworth, Mary L., Latimer, Laura J.P.
Format: Article
Language:English
Subjects:
5-Methylcytosine
8-Bromoguanine
Analytical, structural and metabolic biochemistry
Animals
Antibodies, Monoclonal - immunology
Antibody Specificity
Antigenic determinant
Antigens - immunology
Binding, Competitive
Biological and medical sciences
Bromine
DNA - immunology
Dna, deoxyribonucleoproteins
Female
Fundamental and applied biological sciences. Psychology
Hybridomas - immunology
Mice
Mice, Inbred C57BL
Monoclonal antibody
Nucleic Acid Conformation
Nucleic acids
Osmolar Concentration
Polydeoxyribonucleotides - immunology
Z-DNA
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Both brominated poly[d(GC)] and poly[d(Gm 5C)] form stable left-handed Z-DNA structures at physiological ionic strengths. These two antigens were use to prepare monoclonal antibodies from immunized mice. The specificity of the antibodies was studied in detail with a solid-phase radioimmune assay as well as by means of competition experiments. Both immunogens produced several relatively non-specific antibodies but two types of very specific antibody were also distinguished. The first binds poly[d(Gm 5C)] but not brominated poly[d(GC)] while the other has the opposite specificity and will only bind the brominated polymer.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(84)80267-7