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Functional groups on ‘Z’ DNA recognized by monoclonal antibodies
Both brominated poly[d(GC)] and poly[d(Gm 5C)] form stable left-handed Z-DNA structures at physiological ionic strengths. These two antigens were use to prepare monoclonal antibodies from immunized mice. The specificity of the antibodies was studied in detail with a solid-phase radioimmune assay as...
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| Published in: | FEBS letters 1984-03, Vol.168 (2), p.303-306 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c5267-afa46f660218136ec1d4bc349a9d3422db1f4abe140e3265cc5e64a91e4f29a03 |
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| cites | cdi_FETCH-LOGICAL-c5267-afa46f660218136ec1d4bc349a9d3422db1f4abe140e3265cc5e64a91e4f29a03 |
| container_end_page | 306 |
| container_issue | 2 |
| container_start_page | 303 |
| container_title | FEBS letters |
| container_volume | 168 |
| creator | Lee, Jeremy S. Woodsworth, Mary L. Latimer, Laura J.P. |
| description | Both brominated poly[d(GC)] and poly[d(Gm
5C)] form stable left-handed Z-DNA structures at physiological ionic strengths. These two antigens were use to prepare monoclonal antibodies from immunized mice. The specificity of the antibodies was studied in detail with a solid-phase radioimmune assay as well as by means of competition experiments. Both immunogens produced several relatively non-specific antibodies but two types of very specific antibody were also distinguished. The first binds poly[d(Gm
5C)] but not brominated poly[d(GC)] while the other has the opposite specificity and will only bind the brominated polymer. |
| doi_str_mv | 10.1016/0014-5793(84)80267-7 |
| format | article |
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5C)] but not brominated poly[d(GC)] while the other has the opposite specificity and will only bind the brominated polymer.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(84)80267-7</identifier><identifier>PMID: 6723954</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>5-Methylcytosine ; 8-Bromoguanine ; Analytical, structural and metabolic biochemistry ; Animals ; Antibodies, Monoclonal - immunology ; Antibody Specificity ; Antigenic determinant ; Antigens - immunology ; Binding, Competitive ; Biological and medical sciences ; Bromine ; DNA - immunology ; Dna, deoxyribonucleoproteins ; Female ; Fundamental and applied biological sciences. Psychology ; Hybridomas - immunology ; Mice ; Mice, Inbred C57BL ; Monoclonal antibody ; Nucleic Acid Conformation ; Nucleic acids ; Osmolar Concentration ; Polydeoxyribonucleotides - immunology ; Z-DNA</subject><ispartof>FEBS letters, 1984-03, Vol.168 (2), p.303-306</ispartof><rights>1984</rights><rights>FEBS Letters 168 (1984) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5267-afa46f660218136ec1d4bc349a9d3422db1f4abe140e3265cc5e64a91e4f29a03</citedby><cites>FETCH-LOGICAL-c5267-afa46f660218136ec1d4bc349a9d3422db1f4abe140e3265cc5e64a91e4f29a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579384802677$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3547,27923,27924,45779</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9720401$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6723954$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Jeremy S.</creatorcontrib><creatorcontrib>Woodsworth, Mary L.</creatorcontrib><creatorcontrib>Latimer, Laura J.P.</creatorcontrib><title>Functional groups on ‘Z’ DNA recognized by monoclonal antibodies</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Both brominated poly[d(GC)] and poly[d(Gm
5C)] form stable left-handed Z-DNA structures at physiological ionic strengths. These two antigens were use to prepare monoclonal antibodies from immunized mice. The specificity of the antibodies was studied in detail with a solid-phase radioimmune assay as well as by means of competition experiments. Both immunogens produced several relatively non-specific antibodies but two types of very specific antibody were also distinguished. The first binds poly[d(Gm
5C)] but not brominated poly[d(GC)] while the other has the opposite specificity and will only bind the brominated polymer.</description><subject>5-Methylcytosine</subject><subject>8-Bromoguanine</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibody Specificity</subject><subject>Antigenic determinant</subject><subject>Antigens - immunology</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>Bromine</subject><subject>DNA - immunology</subject><subject>Dna, deoxyribonucleoproteins</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hybridomas - immunology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Monoclonal antibody</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic acids</subject><subject>Osmolar Concentration</subject><subject>Polydeoxyribonucleotides - immunology</subject><subject>Z-DNA</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><recordid>eNqNkc1O3DAUhS1EBVPgDUDKAlVlEfBfHHtTib9pKyHYwIaN5Tg3yChjT-1Jq2HFY8Dr8SQkM6NZUlaW7_nuvcfHCO0TfEwwEScYE54XpWLfJT-SmIoyLzfQiMiS5YwLuYlGa2QbfU3pEfd3SdQW2hIlZargI3Qx7rydueBNmz3E0E1TFnz29vxy__b8ml1cn2YRbHjw7gnqrJpnk-CDbRe48TNXhdpB2kVfGtMm2FudO-hufHl7_iu_uvn5-_z0KrfF4M40hotGCEx7F0yAJTWvLOPKqJpxSuuKNNxUQDgGRkVhbQGCG0WAN1QZzHbQt-XcaQx_OkgzPXHJQtsaD6FLWhJcCiLpf0HCpBJqAfIlaGNIKUKjp9FNTJxrgvWQsh4i1EOEWnK9SFmXfdvBan5XTaBeN61i7fXDlW6SNW0TjbcurTFVUswx6bHxEvvnWph_arUeX57RQRjqki-qg58fy0HQp__XQdTJOvAWatd_30zXwX38oHd0u6xB</recordid><startdate>19840326</startdate><enddate>19840326</enddate><creator>Lee, Jeremy S.</creator><creator>Woodsworth, Mary L.</creator><creator>Latimer, Laura J.P.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19840326</creationdate><title>Functional groups on ‘Z’ DNA recognized by monoclonal antibodies</title><author>Lee, Jeremy S. ; Woodsworth, Mary L. ; Latimer, Laura J.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5267-afa46f660218136ec1d4bc349a9d3422db1f4abe140e3265cc5e64a91e4f29a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>5-Methylcytosine</topic><topic>8-Bromoguanine</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibody Specificity</topic><topic>Antigenic determinant</topic><topic>Antigens - immunology</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>Bromine</topic><topic>DNA - immunology</topic><topic>Dna, deoxyribonucleoproteins</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. 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| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1984-03, Vol.168 (2), p.303-306 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81076182 |
| source | ScienceDirect |
| subjects | 5-Methylcytosine 8-Bromoguanine Analytical, structural and metabolic biochemistry Animals Antibodies, Monoclonal - immunology Antibody Specificity Antigenic determinant Antigens - immunology Binding, Competitive Biological and medical sciences Bromine DNA - immunology Dna, deoxyribonucleoproteins Female Fundamental and applied biological sciences. Psychology Hybridomas - immunology Mice Mice, Inbred C57BL Monoclonal antibody Nucleic Acid Conformation Nucleic acids Osmolar Concentration Polydeoxyribonucleotides - immunology Z-DNA |
| title | Functional groups on ‘Z’ DNA recognized by monoclonal antibodies |
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