Application of the avidin-biotin-peroxidase complex (ABC) technique for detecting avian reovirus in chickens

An immunoperoxidase technique, avidin-biotin-peroxidase complex (ABC), was used for detecting avian reovirus in glutaraldehyde-formalin-fixed, paraffin-embedded tissues. A peroxidase-positive reaction, characterized by the presence of dark brown granules in the cytoplasm of affected cells, was found...

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Bibliographic Details
Published in:Avian diseases 1987-07, Vol.31 (3), p.591-596
Main Authors: Tang, K.N, Fletcher, O.J
Format: Article
Language:English
Subjects:
Animals
Antibodies
ANTIGENE
ANTIGENOS
ANTIGENS
Antigens, Viral - analysis
Avidin
Biotin
Bursa of fabricius
Bursa of Fabricius - microbiology
CHICKENS
Cytoplasm
Guinea Pigs
IMMUNOENZYME TECHNIQUES
Kupffer cells
Laboratory staining techniques
Liver
Liver - microbiology
Macrophages
POLLO
POULET
Poultry Diseases - diagnosis
REOVIRIDAE
Reoviridae - immunology
Reoviridae - isolation & purification
Reoviridae Infections - diagnosis
Reoviridae Infections - veterinary
TECHNIQUE IMMUNOENZYMATIQUE
TECNICAS INMUNOENZIMATICAS
TEJIDO (ANATOMIA)
TISSU
TISSUES
Viruses
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Summary:An immunoperoxidase technique, avidin-biotin-peroxidase complex (ABC), was used for detecting avian reovirus in glutaraldehyde-formalin-fixed, paraffin-embedded tissues. A peroxidase-positive reaction, characterized by the presence of dark brown granules in the cytoplasm of affected cells, was found in the liver (Kupffer cells, macrophages, and hepatocytes) and bursa of Fabricius (epithelial cells, stromal cells, and/or macrophages). The early presence of peroxidase activity in the bursa of Fabricius supports our previous idea that the bursa of Fabricius may serve as a primary site of replication for natural reovirus infection. /// Se usó la técnica de la inmunoperoxidasa con el complejo avidina-biotina-peroxidasa para detectar reovirus aviares en tejidos fijados en glutaraldehído y formol e incluídos en parafina. La reacción positiva a la peroxidasa se caracterizó por la presencia de gránulos pardo-oscuro en el citoplasma de las células afectadas presentes en el hígado (células de Kupffer, macrófagos y hepatocitos) y en la bolsa de Fabricio (células epiteliales y del estroma y/o en los macrófagos).
ISSN:0005-2086
1938-4351
DOI:10.2307/1590745