NMR studies of the stable mismatch purine-thymine in the self-complementary d(CGPuAATTTCG) duplex in solution

One- and two-dimensional nuclear Overhauser effect experiments demonstrate that a single hydrogen bond between a T imino proton and purine N3 is sufficient to hold the base pair dPu.dT in d(CGPuAATTTCG) by a Watson-Crick fashion rather than a Hoogsteen type. In addition, the dPu.dT base pair is well...

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Bibliographic Details
Published in:Biochemistry (Easton) 1987-09, Vol.26 (18), p.5646-5650
Main Authors: Ikuta, Satoshi, Eritja, Ramon, Kaplan, Bruce E, Itakura, Keiichi
Format: Article
Language:English
Subjects:
Base Composition
Base Sequence
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Hydrogen Bonding
Magnetic Resonance Spectroscopy - methods
Molecular biophysics
Nucleic Acid Conformation
Oligonucleotides
Spectroscopy : techniques and spectras
Thymine
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Summary:One- and two-dimensional nuclear Overhauser effect experiments demonstrate that a single hydrogen bond between a T imino proton and purine N3 is sufficient to hold the base pair dPu.dT in d(CGPuAATTTCG) by a Watson-Crick fashion rather than a Hoogsteen type. In addition, the dPu.dT base pair is well stacked with neighboring base pairs. The spin-lattice relaxation measurements at 30 and 35 degrees C of two decamers, d(CGPuAATTTCG) and d(CGAAATTTCG), reveal that the elimination of two single hydrogen bonds of dA.dT base pairs (due to the substitution of adenine for purine) in the sequence results in an increase in the overall imino proton exchange rate from 7 to 36 s-1 at the site of mismatch.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00392a010