Regulation of growth and differentiation of human keratinocytes by type β transforming growth factor and epidermal growth factor

The role of type beta transforming growth factor (TGF beta) and epidermal growth factor (EGF) as regulators of the growth and differentiation of cultured human neonatal epidermal cells and squamous carcinoma cells was investigated in postconfluent cultures. Neither cell proliferation nor DNA synthes...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1987-12, Vol.47 (24), p.6705-6709
Main Authors: REISS, M, SARTORELLI, A. C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Differentiation - drug effects
Cell Division - drug effects
Dermatology
DNA Replication - drug effects
Epidermal Growth Factor - pharmacology
Epidermis - cytology
Epidermis - drug effects
Humans
Keratins
Medical sciences
Peptides - pharmacology
Protein Precursors - analysis
Transforming Growth Factors
Tumors of the skin and soft tissue. Premalignant lesions
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Summary:The role of type beta transforming growth factor (TGF beta) and epidermal growth factor (EGF) as regulators of the growth and differentiation of cultured human neonatal epidermal cells and squamous carcinoma cells was investigated in postconfluent cultures. Neither cell proliferation nor DNA synthesis was affected by treatment with TGF beta alone; however, EGF significantly stimulated cell growth, and this process was specifically antagonized by TGF beta. In addition, TGF beta inhibited the maturation of human foreskin-derived epidermal cells, as measured by their competence to synthesize involucrin and to form cornified cell envelopes, in a dose-dependent manner. Although treatment with EGF did not affect the maturation of human foreskin-derived epidermal cells, the combination of a low concentration of TGF beta with EGF resulted in significant enhancement of the maturation of these normal keratinocytes. Growth of three of four squamous carcinomas in the presence of EGF was not inhibited by TGF beta. In addition, all four carcinomas were either totally or partially resistant to the induction of maturation by the combination of TGF beta and EGF. This resistance of squamous carcinomas to TGF beta was paralleled by an increased sensitivity to the antikeratinizing effects of EGF. Thus, TGF beta inhibited the mitogenic stimulation of keratinocytes by EGF and induces cell maturation.
ISSN:0008-5472
1538-7445