A monoclonal antibody against a synthetic peptide reveals common structures among spectrins and α-actinin

A monoclonal antibody (Mab) against a synthetic peptide, SEDYGKDL, corresponding to one conserved sequence in the chicken α-fodrin repeats reacts in immunoblotting with avian α-spectrin and α-fodrin, both mammalian spectrins and with mammalian α-fodrin. This Mab also reacts with α-actinin in both ch...

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Bibliographic Details
Published in:FEBS letters 1987-11, Vol.224 (1), p.156-160
Main Authors: Närvänen, Outi, Närvänen, Ale, Wasenius, Veli-Matti, Partanen, Paul, Virtanen, Ismo
Format: Article
Language:English
Subjects:
Actinin - immunology
alpha -actinin
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Animals
Antibodies, Monoclonal - immunology
Antibody
Biological and medical sciences
Carrier Proteins - immunology
Chick Embryo
Cross Reactions
Cytoskeleton
Epitopes - immunology
Fundamental and applied biological sciences. Psychology
Glycoproteins
Humans
Microfilament Proteins - immunology
Peptides - chemical synthesis
Peptides - immunology
Phylogeny
Proteins
Sequence Homology, Nucleic Acid
Spectrin
Spectrin - immunology
spectrins
Synthetic peptide
α-Actinin
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Summary:A monoclonal antibody (Mab) against a synthetic peptide, SEDYGKDL, corresponding to one conserved sequence in the chicken α-fodrin repeats reacts in immunoblotting with avian α-spectrin and α-fodrin, both mammalian spectrins and with mammalian α-fodrin. This Mab also reacts with α-actinin in both chicken and human cells. Our results confirm the previously detected structural homology between spectrins and α-actinin and implicate their common evolutionary origin.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(87)80440-4