Determination of Amphetamine, Norephedrine, and Their Phenolic Metabolites in Rat Brain by Gas Chromatography

A specific analytical procedure for the quantitation of amphetamine (I), norephedrine (III), and their amphoteric metabolites, p-hydroxy-amphetamine (II) and p-hydroxynorephedrine (IV), in biological samples using electron-capture gas chromatography (GC-EC) is described. The procedure utilizes the i...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pharmaceutical sciences 1984-06, Vol.73 (6), p.808-812
Main Authors: Coutts, Ronald T., Prelusky, Daniel B., Baker, Glen B.
Format: Article
Language:English
Subjects:
amphetamine
Amphetamine - analysis
Amphetamine-rat brain metabolism
Amphetamine—rat brain metabolism, norephedrine, phenolic metabolites, gas chromatography
Analysis
Animals
Biological and medical sciences
Biotransformation
Brain Chemistry
Chromatography, Gas - methods
gas chro-matography
gas chromatography
General pharmacology
Male
Medical sciences
Metabolites
Metabolites, phenolic—amphetamine and norephedrine, rat brain metabolism, gas chro-matography
norephedrine
Norephedrine-rat brain metabolites
Norephedrine—rat brain metabolites, amphetamine, phenolic metabolites, gas chromatography
p-Hydroxyamphetamine - analysis
p-Hydroxynorephedrine - analysis
Pharmacology. Drug treatments
phenolic metabolites
phenolic-amphetamine and norephedrine
Phenylpropanolamine - analysis
rat brain metabolism
Rats
Rats, Inbred Strains
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A specific analytical procedure for the quantitation of amphetamine (I), norephedrine (III), and their amphoteric metabolites, p-hydroxy-amphetamine (II) and p-hydroxynorephedrine (IV), in biological samples using electron-capture gas chromatography (GC-EC) is described. The procedure utilizes the ion-pairing reagent, bis(2-ethylhexyl)phosphoric acid, which frees the amines from most contaminants and permits the efficient extraction of the amphoteric compounds (as acetates) from the aqueous solution. Amines I and III and acetylated amines II and IV were perfluoro-acetylated prior to GC-EC analysis. Metabolism of I, II, and III in the rat brain was studied. Results indicate that both in vivo and in vitro amines I and III are p-hydroxylated to II and IV, respectively, and II is β-hydroxylated to give IV. Norephedrine (III) was not detected as a rat brain metabolite of amphetamine (I).
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600730625