Approaches to the Development of New Poliovirus Vaccines Based on Molecular Genetics

Complete nucleotide sequences of virion RNAs from immunologically distinct vaccine strains of poliovirus (i.e., Sabin strains 1, 2, and 3) were determined by use of molecular cloning and rapid sequence analysis techniques. When the nucleotide sequences were compared, extensive sequence homology was...

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Bibliographic Details
Published in:Reviews of infectious diseases 1984-05, Vol.6, p.S494-S498
Main Authors: Nomoto, Akio, Toyoda, Haruka, Kataoka, Yutaka, Kohara, Michinori, Toshiyuki Suganuma, Omata, Toshiko, Imura, Nobumasa
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Antigens, Viral - immunology
Base Sequence
Biological and medical sciences
Biotechnology
DNA
Fundamental and applied biological sciences. Psychology
Genomes
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
Nucleotide sequences
Nucleotides
Poliovirus
Poliovirus - genetics
Poliovirus Vaccine, Inactivated
Production of active biomolecules
RNA
Sequence analysis
Session IV: Current Research
Vaccins
Viral Proteins - analysis
Virions
Virulence
Viruses
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Summary:Complete nucleotide sequences of virion RNAs from immunologically distinct vaccine strains of poliovirus (i.e., Sabin strains 1, 2, and 3) were determined by use of molecular cloning and rapid sequence analysis techniques. When the nucleotide sequences were compared, extensive sequence homology was observed among the virion RNAs in the 5′ and 3′ terminal regions. This result strongly suggested that the serotypes of poliovirus were derived from a putative prototype poliovirus and that the homologous sequences conserved through a long evolutionary process may have essential roles for the replication and the viral functions of the virus. Many different amino acids, predicted from the nucleotide sequences, were located in polypeptides P1-1a, especially in P1-VP1, which is located on the surface of the virion and carries viral antigens. Locations of different amino acids, together with the calculated hydrophilicity values in P1-VP1, revealed possible loci for type-specific antigenic determinants. These data on functionally important conserved sequences and on possible locations of antigenic determinants will be useful in the construction of new polio vaccine strains.
ISSN:0162-0886