Interferon Action: Inhibition of Vesicular Stomatitis Virus RNA Synthesis Induced by Virion-Bound Polymerase

The particle-bound RNA polymerase activity of vesicular stomatitis virus (VSV) can be demonstrated in vivo. Linear synthesis of viral RNA persists for 5 to 6 hours at 34°C in infected monolayers of chick embryo cells treated with cycloheximide and actinomycin D to block synthesis of protein and cell...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1971-11, Vol.174 (4009), p.593-598
Main Authors: Marcus, Philip I., Engelhardt, Dean L., Hunt, John M., Sekellick, Margaret J.
Format: Article
Language:English
Subjects:
Actinomycin
Animals
Autoradiography
Cells, Cultured - metabolism
Chick Embryo
Cycloheximide - pharmacology
Dactinomycin - pharmacology
Embryonic cells
Embryos
Immunocompetent cells
Interferons
Interferons - pharmacology
Microscopy, Phase-Contrast
RNA
RNA Nucleotidyltransferases - pharmacology
RNA, Viral - analysis
RNA, Viral - biosynthesis
Surface antigens
Time Factors
Tritium
Uridine - metabolism
Vesicular stomatitis Indiana virus - enzymology
Vesicular stomatitis Indiana virus - metabolism
Viral RNA
Virions
Viruses
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Summary:The particle-bound RNA polymerase activity of vesicular stomatitis virus (VSV) can be demonstrated in vivo. Linear synthesis of viral RNA persists for 5 to 6 hours at 34°C in infected monolayers of chick embryo cells treated with cycloheximide and actinomycin D to block synthesis of protein and cell-specific RNA. At least 55 percent of the RNA made under these conditions is complementary to virion RNA. RNA synthesis mediated by VSV polymerase activity is inhibited in cells first treated with chick-derived interferon or polyriboinosinate·polyribocytidylate, but not by mouse interferon. The RNA product of VSV polymerase activity is present throughout the cytoplasm, and its synthesis is inhibited by the interferon system, as judged by autoradiographs that show the physical distribution, in cells, of RNA produced by virion polymerase in the absence of translation-a demonstration of the transcription product of the viral genome.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.174.4009.593