High-resolution electron microscopy of octacalcium phosphate and its hydrolysis products

The hydrolysis and dehydration products of synthetic octacalcium phosphate (OCP) were studied using X-ray diffraction, infrared spectroscopy, chemical analysis, and high-resolution electron microscopy (HREM). A "collapsed OCP" phase, identified by a characteristic 16.5 A reflection in its...

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Bibliographic Details
Published in:Calcified tissue international 1984-12, Vol.36 (2), p.219-232
Main Authors: NELSON, D. G. A, MCLEAN, J. D
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Apatites - analysis
Biological and medical sciences
Calcium - analysis
Calcium Phosphates - analysis
Crystallography
Fundamental and applied biological sciences. Psychology
Hydrolysis
Inorganic compounds
Microscopy, Electron - methods
Other biological molecules
Phosphorus - analysis
Spectrophotometry, Infrared
Water - analysis
X-Ray Diffraction
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Summary:The hydrolysis and dehydration products of synthetic octacalcium phosphate (OCP) were studied using X-ray diffraction, infrared spectroscopy, chemical analysis, and high-resolution electron microscopy (HREM). A "collapsed OCP" phase, identified by a characteristic 16.5 A reflection in its X-ray diffraction pattern, was observed when OCP was dehydrated. High resolution electron microscopy of the hydrolyzed and partially hydrolyzed reaction products also revealed local contrast features with an approximate 16.5 A periodicity. These features were consistent with a collapse of the OCP crystal structure and subsequent formation of epitaxial intergrowths of OCP and hydroxyapatite. Chemical analysis and X-ray diffraction of these samples were similar to previously reported calcium-deficient apatites. The hydrolysis of OCP to form calcium-deficient apatites is a reaction pathway which may be of importance in understanding the crystallographic changes occurring during the early stages of bone, calculus, and dental enamel formation.
ISSN:0171-967X
1432-0827
DOI:10.1007/bf02405321