“De novo” Synthesis of Cellulase Induced by Sophorose in Trichoderma viride Cells

• Published in: Journal of biochemistry (Tokyo) 1971-09, Vol.70 (3), p.387-393
• Main Authors: NISIZAWA, Tosiki, SUZUKI, Hiroshi, NISIZAWA, Kazutosi
• Format: Article
• Language: English
• Subjects: Amylases - analysis; Carbon Isotopes; Cellulose; Chromatography; Chromatography, Ion Exchange; Disaccharides - pharmacology; Enzyme Induction; Glucosidases; Glycoside Hydrolases - analysis; Leucine; Mitosporic Fungi - enzymology; Phosphoric Monoester Hydrolases; Tritium
• ISSN: 0021-924X; 1756-2651
• DOI: 10.1093/oxfordjournals.jbchem.a129653

The formation of cellulase [EC 3. 2.1.4], β-glucosidase (possibly an exo-type cellulase) [EC 3.2.1.21] and xylanase [EC 3.2.1.8] in washed mycelia of Trichoderma viride was markedly stimulated by sophorose. By contrast, the formation of amylase [EC 3.2.1.1], acid phosphatase [EC 3.1.3.2] and protease was not affected by sophorose. Extracellular and cell-bound enzyme solutions, which were prepared from mycelial suspensions after shaking them with sophorose and L-leucine-14C and only with L-leucine-3H, were fractionated by column chromatography. The major fractions of cellulase and amylase activities from the extracellular enzyme preparations overlapped with each other, and they were chromatographed on a starch column to separate from each other. Almost all of the cellulase activity was recovered in the eluates, and seemed to be labelled only with 14C-radioactivity, while amylase was labelled with both 14C and 3H. The formation of the cell-bound cellulase was similarly affected by sophorose.