Isolation of the gene encoding pilin of Bacteroides nodosus (strain 198), the causal organism of ovine footrot

The gene for pilin, the monomeric protein subunit from which the pilus of Bacteroides nodosus is constructed, has been isolated. Isolation was achieved by cloning the fragmented genome of B. nodosus in Escherichia coli RR1 using the plasmid vector pBR322. Pilin-producing colonies were identified by...

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Bibliographic Details
Published in:FEBS letters 1984-07, Vol.173 (1), p.103-107
Main Authors: Elleman, T.C., Hoyne, P.A., Emery, D.L., Stewart, D.J., Clark, B.L.
Format: Article
Language:English
Subjects:
Animals
Bacterial Proteins - genetics
Bacteriology
Bacteroides - genetics
Bacteroides - ultrastructure
Bacteroides nodosus
Base Sequence
Biological and medical sciences
Cloning
Cloning, Molecular
Fimbriae Proteins
Fimbriae, Bacterial
Foot Rot - microbiology
Footrot
Fundamental and applied biological sciences. Psychology
Genes
Genes, Bacterial
Genetics
kbp, kilobase pair
Membrane Proteins - genetics
Microbiology
PAGE, polyacrylamide gel electrophoresis
Pilin
Sheep
Sheep Diseases - microbiology
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Summary:The gene for pilin, the monomeric protein subunit from which the pilus of Bacteroides nodosus is constructed, has been isolated. Isolation was achieved by cloning the fragmented genome of B. nodosus in Escherichia coli RR1 using the plasmid vector pBR322. Pilin-producing colonies were identified by screening with a colony immunoassay using antiserum from a sheep immunized against purified pili from B. nodosus strain 198, and were further characterized by immunoblot analysis. Final confirmation of the presence of the pilin gene was by nucleotide sequence data which translated to the known pilin amino acid sequence.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(84)81026-1