Effects of VIP and forskolin on alanine metabolism in isolated hepatocytes

• Published in: FEBS letters 1984-08, Vol.173 (2), p.407-413
• Main Authors: Leiser, Jeffrey, Blum, J.J.
• Format: Article
• Language: English
• Subjects: Alanine - metabolism; Aminoacids, peptides. Hormones. Neuropeptides; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Colforsin; Diterpenes - pharmacology; DMSO, dimethyl sulfoxide; Fasting; Fundamental and applied biological sciences. Psychology; Glucagon - pharmacology; Gluconeogenesis - drug effects; In Vitro Techniques; Kinetics; Liver - drug effects; Liver - metabolism; Male; Proteins; Rats; Rats, Inbred Strains; Urea - metabolism; Vasoactive Intestinal Peptide - pharmacology; VIP,vasoactive intestinal polypeptide
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(84)80815-7

The effects of vasoactive intestinal polypeptide (VIP) and of forskolin on alanine metabolism in hepatocytes isolated from fed and fasted rats were examined. VIP and 17 μM forskolin stimulated glucose production, gluconeogenesis from alanine, and ureagenesis, and inhibited glyconeogenesis to comparable degrees. However, combination of 17 μM forskolin with a maximal dose of VIP significantly augmented only the inhibition of glyconeogenesis. At 100 μM, forskolin induced metabolic responses comparable to those induced by glucagon, but similarly, in combination with maximal doses of VIP or glucagon, augmented only inhibition of glycogen synthesis. In addition to demonstrating modulation of alanine metabolism by VIP and forskolin, these results raise questions about the nature of the coupling between VIP receptor occupancy and metabolic response. Vasoactive intestinal polypeptide Forskolin Gluconeogenesis Alanine Glycogen metabolism