In Vitro Study on Contribution of Oxidative Metabolism of Isolated Rabbit Heart Mitochondria to Myocardial Reperfusion Injury

The present study was performed to clarify oxygen-induced damage following myocardial reperfusion, using three mitochondrial preparations from isolated rabbit hearts (non-ischemic hearts and those subjected to 40 and 90 minutes of normothermic global ischemia). The viability of mitochondria was eval...

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Bibliographic Details
Published in:Circulation research 1984-08, Vol.55 (2), p.168-175
Main Authors: Otani, Hajime, Tanaka, Hidehiko, Inoue, Takeo, Umemoto, Masao, Omoto, Kazuo, Tanaka, Kazuho, Sato, Tadashi, Osako, Tsutomu, Masuda, Atoh, Nonoyama, Akira, Kagawa, Terumasa
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Animals
Biological and medical sciences
Cardiology. Vascular system
Catalase - metabolism
Coenzymes
Coronary heart disease
Electron Spin Resonance Spectroscopy
Female
Free Radicals
Heart
Hydroxides
Hydroxyl Radical
Male
Medical sciences
Mitochondria, Heart - metabolism
Myocardium - pathology
Oxygen Consumption
Perfusion
Rabbits
Superoxide Dismutase - metabolism
Time Factors
Ubiquinone - analogs & derivatives
Ubiquinone - metabolism
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Summary:The present study was performed to clarify oxygen-induced damage following myocardial reperfusion, using three mitochondrial preparations from isolated rabbit hearts (non-ischemic hearts and those subjected to 40 and 90 minutes of normothermic global ischemia). The viability of mitochondria was evaluated by adenosine triphosphate generation. The extent of mitochondrial injury produced by reactive oxygen metabolism was assessed by the intensity of hydroxyl radical signal detected with electron spin resonance spectroscopy and the reduction of coenzyme Q10 level. The greatest oxygen-induced injury was observed in 40-minute ischemic mitochondria exposed to pure oxygen. The use of superoxide dismutase and catalase satisfactorily prevented the oxygen-induced injury. Moreover, the net adenosine triphosphate generation of the 40-minute ischemic mitochondria was comparable to that of the nonischemic mitochondria without the enzymes. These results suggest that reperfusion of the ischemic myocardium with viable mitochondria is deleterious, because mitochondria are susceptible to injury resulting from oxidative metabolism, and that the use of superoxide dismutase, together with catalase, is beneficial for the restoration of cardiac function after ischemia.
ISSN:0009-7330
1524-4571
DOI:10.1161/01.res.55.2.168