Streptomycin retards the phenotypic maturation of chick myogenic cells [Tissue culture]

As part of an effort to optimize conditions required for the complete maturation of muscle cells in vitro, we have investigated the effects of the antibiotics penicillin, streptomycin, and Fungizone (amphotericin B) on the development of cultured chick embryo skeletal muscle. It is shown that even l...

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Bibliographic Details
Published in:In Vitro 1984-06, Vol.20 (6), p.473-478
Main Authors: Moss, P.S, Spector, D.H, Glass, C.A, Strohman, R.C
Format: Article
Language:English
Subjects:
Amphotericin B - pharmacology
Animal cells
Animals
Antibiotics
Biological and medical sciences
Biotechnology
Cell culture techniques
Cell cultures. Hybridization. Fusion
Cell Differentiation - drug effects
Cell fusion
Cell growth
Cells, Cultured
Chick Embryo
Cultured cells
Dose-Response Relationship, Drug
Embryos
Establishment of new cell lines, improvement of cultural methods, mass cultures
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Molecular and cellular biology
Muscles - cytology
Myosins - biosynthesis
Neurons
Penicillin
Penicillins - pharmacology
Phenotype
Protein synthesis
Skeletal muscle
Streptomycin - pharmacology
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Summary:As part of an effort to optimize conditions required for the complete maturation of muscle cells in vitro, we have investigated the effects of the antibiotics penicillin, streptomycin, and Fungizone (amphotericin B) on the development of cultured chick embryo skeletal muscle. It is shown that even low dosages of streptomycin, but not penicillin or Fungizone, retard protein synthesis and accumulation in these cultures. Myosin accumulation was also reduced and the appearance of striations in fused cells was delayed in myotubes formed in medium containing streptomycin. Additional data suggest that this overall retardation of myogenesis is due to the influence of streptomycin on maturing myotubes rather than early proliferation and cell fusion. These results are discussed with regard to recent efforts to promote the full maturation of muscle cells grown in culture.
ISSN:0073-5655
2327-4328
1475-2689
DOI:10.1007/BF02619620