Human Cytomegalovirus DNA Sequences with Homologies to the Cellular Genome

Institut für Klinische Virologie, Universität Erlangen-Nürnberg, D-8520 Erlangen and 1 Institut für Virologie der Universität Freiburg, D-7800 Freiburg, Federal Republic of Germany DNA from a cosmid-cloned gene library of human cytomegalovirus (HCMV) strain AD169 was found to share sequence homologi...

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Bibliographic Details
Published in:Journal of general virology 1984-08, Vol.65 (8), p.1351-1364
Main Authors: Ruger, Rudiger, Bornkamm, Georg W, Fleckenstein, Bernhard
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Chromosome Mapping
Cytomegalovirus - genetics
DNA, Viral - genetics
Fundamental and applied biological sciences. Psychology
Genes, Viral
Humans
Microbiology
Morphology, structure, chemical composition, physicochemical properties
Nucleic Acid Hybridization
Oncogenes
Virology
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Summary:Institut für Klinische Virologie, Universität Erlangen-Nürnberg, D-8520 Erlangen and 1 Institut für Virologie der Universität Freiburg, D-7800 Freiburg, Federal Republic of Germany DNA from a cosmid-cloned gene library of human cytomegalovirus (HCMV) strain AD169 was found to share sequence homologies with cellular DNA of various origins. Viral DNA fragments subcloned in plasmid vectors enabled localization of the homologous sequences to five regions of the HCMV genome. Four of these regions were found in the long unique (U L ) segment of the viral genome ( Eco RI fragments R, I and b, and Hin dIII fragment S); one region with virus-cell homology was located in the terminal inverted repeats ( Eco RI fragments O and H). All hybridization reactions were carried out under stringent reannealing conditions (18 °C to 21.5 °C below average T m of HCMV DNA). Fragments from these five HCMV DNA regions did not cross-hybridize with each other. The sequence homologies were seen in DNA from normal human placental tissue, liver autopsy material, intestinal tissue, white blood cells, and colon carcinoma cells, and in a series of haematopoietic cell lines. Sequence homologies were also detected in DNA from owl monkeys and Chinese hamsters, but not in mouse DNA. They were not related to human Alu repeats or cloned human c-myc sequences. Keywords: HCMV, homology, repetitive cell DNA Received 5 January 1984; accepted 27 April 1984.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-65-8-1351