Loading…
Human Cytomegalovirus DNA Sequences with Homologies to the Cellular Genome
Institut für Klinische Virologie, Universität Erlangen-Nürnberg, D-8520 Erlangen and 1 Institut für Virologie der Universität Freiburg, D-7800 Freiburg, Federal Republic of Germany DNA from a cosmid-cloned gene library of human cytomegalovirus (HCMV) strain AD169 was found to share sequence homologi...
Saved in:
| Published in: | Journal of general virology 1984-08, Vol.65 (8), p.1351-1364 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c438t-401cdf89aecdb5f1185f57c74c75abc411b5f496702e79ae457063b5e510cebb3 |
|---|---|
| cites | |
| container_end_page | 1364 |
| container_issue | 8 |
| container_start_page | 1351 |
| container_title | Journal of general virology |
| container_volume | 65 |
| creator | Ruger, Rudiger Bornkamm, Georg W Fleckenstein, Bernhard |
| description | Institut für Klinische Virologie, Universität Erlangen-Nürnberg, D-8520 Erlangen
and 1 Institut für Virologie der Universität Freiburg, D-7800 Freiburg, Federal Republic of Germany
DNA from a cosmid-cloned gene library of human cytomegalovirus (HCMV) strain AD169 was found to share sequence homologies with cellular DNA of various origins. Viral DNA fragments subcloned in plasmid vectors enabled localization of the homologous sequences to five regions of the HCMV genome. Four of these regions were found in the long unique (U L ) segment of the viral genome ( Eco RI fragments R, I and b, and Hin dIII fragment S); one region with virus-cell homology was located in the terminal inverted repeats ( Eco RI fragments O and H). All hybridization reactions were carried out under stringent reannealing conditions (18 °C to 21.5 °C below average T m of HCMV DNA). Fragments from these five HCMV DNA regions did not cross-hybridize with each other. The sequence homologies were seen in DNA from normal human placental tissue, liver autopsy material, intestinal tissue, white blood cells, and colon carcinoma cells, and in a series of haematopoietic cell lines. Sequence homologies were also detected in DNA from owl monkeys and Chinese hamsters, but not in mouse DNA. They were not related to human Alu repeats or cloned human c-myc sequences.
Keywords: HCMV, homology, repetitive cell DNA
Received 5 January 1984;
accepted 27 April 1984. |
| doi_str_mv | 10.1099/0022-1317-65-8-1351 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_81172616</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>13930307</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-401cdf89aecdb5f1185f57c74c75abc411b5f496702e79ae457063b5e510cebb3</originalsourceid><addsrcrecordid>eNqFkU1PxCAQhonR6PrxC4xJD8Z4qTKlFHo068dqjB7UM6HsdBfTFoVW47-XZjfr0RMM7zMzb14IOQZ6AbQsLynNshQYiLTgqYw3DltkAnmssqhvk8mG2CP7IbxTCnnOxS7ZLagsZAYT8jAbWt0l05_etbjQjfuyfgjJ9dNV8oKfA3YGQ_Jt-2Uyc61r3MLGundJv8Rkik0zNNond9jF7kOyU-sm4NH6PCBvtzev01n6-Hx3P716TE3OZJ_mFMy8lqVGM694DSB5zYURuRFcVyYHiK95WQiaoYhUNEwLVnHkQA1WFTsgZ6u5H95Fh6FXrQ0metEduiEoCSCyAop_QWAlo4yKCLIVaLwLwWOtPrxttf9RQNUYtRqDVGOQquBKqjHq2HWyHj9ULc43Petso3661nUwuqm97owNG0xKWUo2Lj9fYUu7WH5bj2qBXWujlco6Fb_jb-MvVUmTnw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>13930307</pqid></control><display><type>article</type><title>Human Cytomegalovirus DNA Sequences with Homologies to the Cellular Genome</title><source>Freely Accessible Journals</source><creator>Ruger, Rudiger ; Bornkamm, Georg W ; Fleckenstein, Bernhard</creator><creatorcontrib>Ruger, Rudiger ; Bornkamm, Georg W ; Fleckenstein, Bernhard</creatorcontrib><description>Institut für Klinische Virologie, Universität Erlangen-Nürnberg, D-8520 Erlangen
and 1 Institut für Virologie der Universität Freiburg, D-7800 Freiburg, Federal Republic of Germany
DNA from a cosmid-cloned gene library of human cytomegalovirus (HCMV) strain AD169 was found to share sequence homologies with cellular DNA of various origins. Viral DNA fragments subcloned in plasmid vectors enabled localization of the homologous sequences to five regions of the HCMV genome. Four of these regions were found in the long unique (U L ) segment of the viral genome ( Eco RI fragments R, I and b, and Hin dIII fragment S); one region with virus-cell homology was located in the terminal inverted repeats ( Eco RI fragments O and H). All hybridization reactions were carried out under stringent reannealing conditions (18 °C to 21.5 °C below average T m of HCMV DNA). Fragments from these five HCMV DNA regions did not cross-hybridize with each other. The sequence homologies were seen in DNA from normal human placental tissue, liver autopsy material, intestinal tissue, white blood cells, and colon carcinoma cells, and in a series of haematopoietic cell lines. Sequence homologies were also detected in DNA from owl monkeys and Chinese hamsters, but not in mouse DNA. They were not related to human Alu repeats or cloned human c-myc sequences.
Keywords: HCMV, homology, repetitive cell DNA
Received 5 January 1984;
accepted 27 April 1984.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-65-8-1351</identifier><identifier>PMID: 6086821</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Base Sequence ; Biological and medical sciences ; Chromosome Mapping ; Cytomegalovirus - genetics ; DNA, Viral - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Viral ; Humans ; Microbiology ; Morphology, structure, chemical composition, physicochemical properties ; Nucleic Acid Hybridization ; Oncogenes ; Virology</subject><ispartof>Journal of general virology, 1984-08, Vol.65 (8), p.1351-1364</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-401cdf89aecdb5f1185f57c74c75abc411b5f496702e79ae457063b5e510cebb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8889837$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6086821$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ruger, Rudiger</creatorcontrib><creatorcontrib>Bornkamm, Georg W</creatorcontrib><creatorcontrib>Fleckenstein, Bernhard</creatorcontrib><title>Human Cytomegalovirus DNA Sequences with Homologies to the Cellular Genome</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Institut für Klinische Virologie, Universität Erlangen-Nürnberg, D-8520 Erlangen
and 1 Institut für Virologie der Universität Freiburg, D-7800 Freiburg, Federal Republic of Germany
DNA from a cosmid-cloned gene library of human cytomegalovirus (HCMV) strain AD169 was found to share sequence homologies with cellular DNA of various origins. Viral DNA fragments subcloned in plasmid vectors enabled localization of the homologous sequences to five regions of the HCMV genome. Four of these regions were found in the long unique (U L ) segment of the viral genome ( Eco RI fragments R, I and b, and Hin dIII fragment S); one region with virus-cell homology was located in the terminal inverted repeats ( Eco RI fragments O and H). All hybridization reactions were carried out under stringent reannealing conditions (18 °C to 21.5 °C below average T m of HCMV DNA). Fragments from these five HCMV DNA regions did not cross-hybridize with each other. The sequence homologies were seen in DNA from normal human placental tissue, liver autopsy material, intestinal tissue, white blood cells, and colon carcinoma cells, and in a series of haematopoietic cell lines. Sequence homologies were also detected in DNA from owl monkeys and Chinese hamsters, but not in mouse DNA. They were not related to human Alu repeats or cloned human c-myc sequences.
Keywords: HCMV, homology, repetitive cell DNA
Received 5 January 1984;
accepted 27 April 1984.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Chromosome Mapping</subject><subject>Cytomegalovirus - genetics</subject><subject>DNA, Viral - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Viral</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Morphology, structure, chemical composition, physicochemical properties</subject><subject>Nucleic Acid Hybridization</subject><subject>Oncogenes</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><recordid>eNqFkU1PxCAQhonR6PrxC4xJD8Z4qTKlFHo068dqjB7UM6HsdBfTFoVW47-XZjfr0RMM7zMzb14IOQZ6AbQsLynNshQYiLTgqYw3DltkAnmssqhvk8mG2CP7IbxTCnnOxS7ZLagsZAYT8jAbWt0l05_etbjQjfuyfgjJ9dNV8oKfA3YGQ_Jt-2Uyc61r3MLGundJv8Rkik0zNNond9jF7kOyU-sm4NH6PCBvtzev01n6-Hx3P716TE3OZJ_mFMy8lqVGM694DSB5zYURuRFcVyYHiK95WQiaoYhUNEwLVnHkQA1WFTsgZ6u5H95Fh6FXrQ0metEduiEoCSCyAop_QWAlo4yKCLIVaLwLwWOtPrxttf9RQNUYtRqDVGOQquBKqjHq2HWyHj9ULc43Petso3661nUwuqm97owNG0xKWUo2Lj9fYUu7WH5bj2qBXWujlco6Fb_jb-MvVUmTnw</recordid><startdate>198408</startdate><enddate>198408</enddate><creator>Ruger, Rudiger</creator><creator>Bornkamm, Georg W</creator><creator>Fleckenstein, Bernhard</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>198408</creationdate><title>Human Cytomegalovirus DNA Sequences with Homologies to the Cellular Genome</title><author>Ruger, Rudiger ; Bornkamm, Georg W ; Fleckenstein, Bernhard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-401cdf89aecdb5f1185f57c74c75abc411b5f496702e79ae457063b5e510cebb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Chromosome Mapping</topic><topic>Cytomegalovirus - genetics</topic><topic>DNA, Viral - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Viral</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Morphology, structure, chemical composition, physicochemical properties</topic><topic>Nucleic Acid Hybridization</topic><topic>Oncogenes</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ruger, Rudiger</creatorcontrib><creatorcontrib>Bornkamm, Georg W</creatorcontrib><creatorcontrib>Fleckenstein, Bernhard</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ruger, Rudiger</au><au>Bornkamm, Georg W</au><au>Fleckenstein, Bernhard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Cytomegalovirus DNA Sequences with Homologies to the Cellular Genome</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1984-08</date><risdate>1984</risdate><volume>65</volume><issue>8</issue><spage>1351</spage><epage>1364</epage><pages>1351-1364</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>Institut für Klinische Virologie, Universität Erlangen-Nürnberg, D-8520 Erlangen
and 1 Institut für Virologie der Universität Freiburg, D-7800 Freiburg, Federal Republic of Germany
DNA from a cosmid-cloned gene library of human cytomegalovirus (HCMV) strain AD169 was found to share sequence homologies with cellular DNA of various origins. Viral DNA fragments subcloned in plasmid vectors enabled localization of the homologous sequences to five regions of the HCMV genome. Four of these regions were found in the long unique (U L ) segment of the viral genome ( Eco RI fragments R, I and b, and Hin dIII fragment S); one region with virus-cell homology was located in the terminal inverted repeats ( Eco RI fragments O and H). All hybridization reactions were carried out under stringent reannealing conditions (18 °C to 21.5 °C below average T m of HCMV DNA). Fragments from these five HCMV DNA regions did not cross-hybridize with each other. The sequence homologies were seen in DNA from normal human placental tissue, liver autopsy material, intestinal tissue, white blood cells, and colon carcinoma cells, and in a series of haematopoietic cell lines. Sequence homologies were also detected in DNA from owl monkeys and Chinese hamsters, but not in mouse DNA. They were not related to human Alu repeats or cloned human c-myc sequences.
Keywords: HCMV, homology, repetitive cell DNA
Received 5 January 1984;
accepted 27 April 1984.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>6086821</pmid><doi>10.1099/0022-1317-65-8-1351</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1317 |
| ispartof | Journal of general virology, 1984-08, Vol.65 (8), p.1351-1364 |
| issn | 0022-1317 1465-2099 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81172616 |
| source | Freely Accessible Journals |
| subjects | Base Sequence Biological and medical sciences Chromosome Mapping Cytomegalovirus - genetics DNA, Viral - genetics Fundamental and applied biological sciences. Psychology Genes, Viral Humans Microbiology Morphology, structure, chemical composition, physicochemical properties Nucleic Acid Hybridization Oncogenes Virology |
| title | Human Cytomegalovirus DNA Sequences with Homologies to the Cellular Genome |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T19%3A10%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Human%20Cytomegalovirus%20DNA%20Sequences%20with%20Homologies%20to%20the%20Cellular%20Genome&rft.jtitle=Journal%20of%20general%20virology&rft.au=Ruger,%20Rudiger&rft.date=1984-08&rft.volume=65&rft.issue=8&rft.spage=1351&rft.epage=1364&rft.pages=1351-1364&rft.issn=0022-1317&rft.eissn=1465-2099&rft.coden=JGVIAY&rft_id=info:doi/10.1099/0022-1317-65-8-1351&rft_dat=%3Cproquest_cross%3E13930307%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c438t-401cdf89aecdb5f1185f57c74c75abc411b5f496702e79ae457063b5e510cebb3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=13930307&rft_id=info:pmid/6086821&rfr_iscdi=true |