Nucleohistone Content during Aging in Tissue Culture

Nucleohistone was extracted from chick embryo cells at intervals during their in vitro life span. Results showed no significant quantitative change in histone-DNA ratios between old and young cells. Young cells exhibited a cyclic variation in histone-DNA ratio that was not detected in aged cells. Hi...

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Bibliographic Details
Published in:In Vitro 1971-01, Vol.6 (4), p.269-273
Main Authors: Jon M. Ryan, Quinn, Loyd Y.
Format: Article
Language:English
Subjects:
Animals
Cell Count
Cell growth
Cell Nucleus - analysis
Cells, Cultured - analysis
Chemical suspensions
Chick Embryo
Chromatin
Diploidy
DNA
DNA - analysis
DNA - isolation & purification
Embryonic cells
Embryos
HeLa cells
Histones
Histones - analysis
Histones - biosynthesis
Histones - isolation & purification
Methods
Mitosis
Proteins - isolation & purification
Spectrophotometry
Steepest descent method
Time Factors
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Description
Summary:Nucleohistone was extracted from chick embryo cells at intervals during their in vitro life span. Results showed no significant quantitative change in histone-DNA ratios between old and young cells. Young cells exhibited a cyclic variation in histone-DNA ratio that was not detected in aged cells. Histone-DNA values throughout the growth cycle for aged cells were similar to those obtained for young cells in stationary phase. This finding suggests that aged cells resemble young cells in stationary phase with respect to histone-DNA ratio.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02625940