‘Young’ Oral Fibroblasts Are Geno/Phenotypically Distinct

Wound healing within the oral mucosa results in minimal scar formation compared with wounds within the skin. We have recently demonstrated distinct differences in the aging profiles of cells (oral mucosal and patient-matched skin fibroblasts) isolated from these tissues. We hypothesized that the inc...

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Bibliographic Details
Published in:Journal of dental research 2010-12, Vol.89 (12), p.1407-1413
Main Authors: Enoch, S., Peake, M.A., Wall, I., Davies, L., Farrier, J., Giles, P., Kipling, D., Price, P., Moseley, R., Thomas, D., Stephens, P.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Case-Control Studies
Cell Count
Cell Culture Techniques
Cell Cycle - physiology
Cell Movement - physiology
Cell Proliferation
Cellular Senescence - physiology
Collagen - metabolism
Culture Media, Conditioned - analysis
Degeneration. Regeneration. Wound healing. Graft
Dentistry
Enzyme Precursors - analysis
Extracellular Matrix - physiology
Fibroblasts - cytology
Fibroblasts - physiology
Fundamental and applied biological sciences. Psychology
Gelatinases - analysis
Gene Expression Profiling
Genotype
Humans
Matrix Metalloproteinase 2 - analysis
Microarray Analysis
Mouth Mucosa - cytology
Mouth Mucosa - injuries
Phenotype
Regeneration - genetics
Regeneration - physiology
Skin - cytology
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Wound Healing - genetics
Wound Healing - physiology
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Summary:Wound healing within the oral mucosa results in minimal scar formation compared with wounds within the skin. We have recently demonstrated distinct differences in the aging profiles of cells (oral mucosal and patient-matched skin fibroblasts) isolated from these tissues. We hypothesized that the increased replicative potential of oral mucosal fibroblasts may confer upon them preferential wound-healing capacities. Passage-matched early cultures of oral mucosal fibroblasts and skin fibroblasts demonstrated distinct gene expression profiles, with several genes linked to wound healing/tissue repair. This was related to an increased ability of the ‘replicatively younger’ oral mucosal fibroblasts to repopulate a wound space and reorganize their surrounding extracellular matrix environment, key activities during the wound-healing process. We conclude that oral mucosal fibroblasts exhibit a preferential healing response in vivo, due to their ‘replicatively younger’ phenotype when compared with that of patient-matched skin fibroblasts.
ISSN:0022-0345
1544-0591
DOI:10.1177/0022034510377796