Quantification of metformin by the HPLC method in brain regions, cerebrospinal fluid and plasma of rats treated with lipopolysaccharide

Recently, it has been reported that metformin may attenuate inflammation and directly act on the central nervous system. Using the HPLC method, in Wistar rats, we assessed the changes in metformin concentrations in various brain regions (pituitary gland, olfactory bulb, hypothalamus, cerebellum, hip...

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Bibliographic Details
Published in:Pharmacological reports 2010-09, Vol.62 (5), p.956-965
Main Authors: Łabuzek, Krzysztof, Suchy, Dariusz, Gabryel, Bożena, Bielecka, Anna, Liber, Sebastian, Okopień, Bogusław
Format: Article
Language:English
Subjects:
Administration, Oral
Animals
Blood-Brain Barrier - metabolism
brain
Brain - metabolism
cerebrospinal fluid
Chromatography, High Pressure Liquid
Dose-Response Relationship, Drug
Drug Safety and Pharmacovigilance
HPLC
Hypoglycemic Agents - blood
Hypoglycemic Agents - cerebrospinal fluid
Hypoglycemic Agents - pharmacokinetics
Inflammation - chemically induced
Inflammation - metabolism
lipopolysaccharide
Lipopolysaccharides - pharmacology
Male
metformin
Metformin - blood
Metformin - cerebrospinal fluid
Metformin - pharmacokinetics
Pharmacotherapy
Pharmacy
plasma
rat
Rats
Rats, Wistar
Short Communication
Tissue Distribution
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Summary:Recently, it has been reported that metformin may attenuate inflammation and directly act on the central nervous system. Using the HPLC method, in Wistar rats, we assessed the changes in metformin concentrations in various brain regions (pituitary gland, olfactory bulb, hypothalamus, cerebellum, hippocampus, striatum, frontal cortex), cerebrospinal fluid and plasma after single and chronic oral administration, in the model of systemic inflammation induced by lipopolysaccharide (ip). Regarding the influence of systemic inflammation on metformin distribution, the pituitary gland demonstrated the highest its level after single and chronic administration (28.8±3.5nmol/g and 24.9±3.2nmol/g, respectively). We concluded that orally-dosed metformin rapidly crosses the blood-brain barrier and differently accumulates in structures of the central nervous system.
ISSN:1734-1140
2299-5684
DOI:10.1016/S1734-1140(10)70357-1