Lipid and Protein Composition of the Surface Tegument from Larvae of Taenia taeniaeformis

A tegumental fraction from fully developed larvae of Taenia taeniaeformis was recovered by low speed centrifugation following incubation of the parasites in a 0.1% solution of digitonin. Scanning electron microscopy of the parasite carcass revealed no surface microtrichs, and transmission electron m...

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Bibliographic Details
Published in:The Journal of parasitology 1984-04, Vol.70 (2), p.197-207
Main Authors: Mills, Gary L., Coley, Susan C., Williams, Jeffrey F.
Format: Article
Language:English
Subjects:
Animals
Cell Biology
Cholesterol - analysis
Concanavalin A - metabolism
Gels
Glycolipids
Glycolipids - analysis
Glycoproteins
Glycoproteins - analysis
Glycosphingolipids - analysis
Larva - analysis
Larvae
Lipids
Lipids - analysis
Microvilli
Molecular Weight
Parasite hosts
Parasites
Parasitism
Phosphatidylethanolamines - analysis
Phosphatidylserines - analysis
Phospholipids
Proteins - analysis
Succinate Dehydrogenase - metabolism
Taenia - analysis
Taenia - metabolism
Taenia - ultrastructure
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Summary:A tegumental fraction from fully developed larvae of Taenia taeniaeformis was recovered by low speed centrifugation following incubation of the parasites in a 0.1% solution of digitonin. Scanning electron microscopy of the parasite carcass revealed no surface microtrichs, and transmission electron microscopy indicated that the subtegumental layer was undamaged. The tegumental fraction, judging from the distribution of 3H-Concanavalin A, was enriched for surface components, exhibited low succinic dehydrogenase activity, and an electron microscopic examination of the pellet showed a slightly expanded but intact distal tegumental layer. The fraction, which made up 3.0% of the dry weight of the parasite, consisted of 52% protein and 32% lipid. Thirty-three proteins, ranging in Mr from 9,000 to 276,000 daltons, were detected after sodium dodecyl sulfate solubilization and polyacrylamide gel electrophoresis. Seven of these proteins were glycoproteins. Cholesterol, phosphatidylethanolamine, phosphatidylserine, and glycosphingolipids were the major lipids.
ISSN:0022-3395
1937-2345
DOI:10.2307/3281862