NAD + kinase: molecular weight determination by low-angle laser-light scattering

Low-angle laser-light scattering (LALLS) was employed to measure the absolute molecular weight of chicken liver NAD + kinase (NADK). The weight-average molecular weight ( M w,) was found to be 275000 ± 15000. The corresponding value for the second virial coefficient was −1.65 × 10 −3 ml·mol·g 2. The...

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Bibliographic Details
Published in:FEBS letters 1984-10, Vol.175 (2), p.419-421
Main Authors: Gabriel, Mina K., McGuinness, Eugene T.
Format: Article
Language:English
Subjects:
Absolute molecular weight
Analytical, structural and metabolic biochemistry
Animals
Azotobacter - enzymology
Biological and medical sciences
Columbidae
Enzymes and enzyme inhibitors
Female
Fundamental and applied biological sciences. Psychology
Fungi - enzymology
Lasers
Liver - enzymology
Low-angle laser-light scattering
Molecular Weight
Muscles - enzymology
NAD+ kinase
Organ Specificity
Ovum - enzymology
Phosphotransferases (Alcohol Group Acceptor)
Phosphotransferases - isolation & purification
Plants - enzymology
Rabbits
Rats
Scattering, Radiation
Sea Urchins
Species Specificity
Transferases
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Summary:Low-angle laser-light scattering (LALLS) was employed to measure the absolute molecular weight of chicken liver NAD + kinase (NADK). The weight-average molecular weight ( M w,) was found to be 275000 ± 15000. The corresponding value for the second virial coefficient was −1.65 × 10 −3 ml·mol·g 2. The value for M w is in close accord with estimates reported for pigeon liver (270000) and C. utilis (260000) NADK. If the active enzyme is a dimer, the weight difference between pigeon/chicken liver and rabbit liver (136000) NADK would indicate that the latter enzyme is an active monomer unit.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(84)80780-2