Isolation of adenosine 5'-diphosphate-L-glycero-D-mannoheptose, the assumed substrate of heptose transferase(s), from Salmonella minnesota R595 and Shigella sonnei Re mutants

From heptose transferase-less Re mutants of Salmonella minnesota and Shigella sonnei, a mixture of nucleotide-linked heptoses was isolated. After paper chromatography in different solvent systems, ADP derivatives of D-glycero-D-mannoheptose and L-glycero-D-mannoheptose could be isolated in pure form...

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Bibliographic Details
Published in:The Journal of biological chemistry 1984-10, Vol.259 (19), p.11858-11860
Main Authors: KOCSIS, B, KONTROHR, T
Format: Article
Language:English
Subjects:
Adenosine Diphosphate Sugars - isolation & purification
Bacteriology
Biological and medical sciences
Chromatography, Gas
Chromatography, Ion Exchange
Fundamental and applied biological sciences. Psychology
Metabolism. Enzymes
Microbiology
Mutation
Nucleoside Diphosphate Sugars - isolation & purification
Salmonella - enzymology
Salmonella - genetics
Shigella sonnei - enzymology
Shigella sonnei - genetics
Transferases - genetics
Transferases - metabolism
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Summary:From heptose transferase-less Re mutants of Salmonella minnesota and Shigella sonnei, a mixture of nucleotide-linked heptoses was isolated. After paper chromatography in different solvent systems, ADP derivatives of D-glycero-D-mannoheptose and L-glycero-D-mannoheptose could be isolated in pure form. The structure of ADP-L-glycero-D-mannoheptose was verified by analytical methods and by transformation of ADP-D-glycero-D-mannoheptose with ADP-D-glycero-D-mannoheptose-6-epimerase.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(20)71290-1