Concentrations and composition of glycosaminoglycans in the female bovine reproductive tract

Our objective was to isolate glycosaminoglycans from various regions of the female bovine reproductive tract to ascertain their concentrations and composition. Oviducts, uterine horns, and cervices from 104 female bovine reproductive tracts obtained at slaughter were flushed with 5 ml phosphate buff...

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Bibliographic Details
Published in:Journal of dairy science 1984-09, Vol.67 (9), p.2006-2009
Main Authors: Lee, C.N, Ax, R.L
Format: Article
Language:English
Subjects:
Animals
Cattle - metabolism
Cervix Mucus - analysis
Cervix Uteri - analysis
Chondroitin Sulfates - analysis
Chromatography, High Pressure Liquid
COWS
Estrus
Fallopian Tubes - analysis
Female
FEMALE GENITALIA
Genitalia, Female - analysis
GLYCOSAMINOGLYCANS
Glycosaminoglycans - analysis
Heparin - analysis
Hyaluronic Acid - analysis
ORGANE REPRODUCTEUR
ORGANOS REPRODUCTORES
Pregnancy
REPRODUCTIVE ORGANS
Uterus - analysis
VACA
VACHE
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Summary:Our objective was to isolate glycosaminoglycans from various regions of the female bovine reproductive tract to ascertain their concentrations and composition. Oviducts, uterine horns, and cervices from 104 female bovine reproductive tracts obtained at slaughter were flushed with 5 ml phosphate buffered saline. Following proteolysis, glycosaminoglycans were isolated by alcoholic precipitation, and concentrations were quantitated by high performance liquid chromatography. Concentrations of glycosaminoglycans decreased significantly anterior to the cervix. There was an increase of chondroitin sulfates and heparin-like material in estrual cervical mucus compared with cervical mucus collected during the luteal phase of the estrous cycle.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.s0022-0302(84)81536-2