Liver Orcein Stain and Viral DNA in Duck Hepatitis B Virus Infection in Chinese Ducks and Experimentally Infected Japanese Ducklings

Liver sections were stained with orcein, and duck hepatitis B virus was identified in sera and livers by the hybridization technique in 106 ducks (44 Chinese ducks, 15 Japanese ducks and 47 Japanese ducklings). Orcein‐positive hepatocytes were found in 18 of 38 (47%) duck hepatitis B virus DNA serop...

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Bibliographic Details
Published in:Hepatology (Baltimore, Md.) Md.), 1984-11, Vol.4 (6), p.1124-1128
Main Authors: Mori, Junko, Omata, Masao, Yokosuka, Osamu, Imazeki, Fumio, Ito, Yoshimi, Uchiumi, Katsuo, Matsuyama, Yasuhisa, Weifa, Ye, Okuda, Kunio
Format: Article
Language:English
Subjects:
Animal viral diseases
Animals
Biological and medical sciences
DNA, Viral - analysis
Ducks - microbiology
Hepatitis B - pathology
Hepatitis B - veterinary
Infectious diseases
Liver - analysis
Liver - pathology
Marmota
Medical sciences
Oxazines
Poultry Diseases - pathology
Staining and Labeling
Viral diseases
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Summary:Liver sections were stained with orcein, and duck hepatitis B virus was identified in sera and livers by the hybridization technique in 106 ducks (44 Chinese ducks, 15 Japanese ducks and 47 Japanese ducklings). Orcein‐positive hepatocytes were found in 18 of 38 (47%) duck hepatitis B virus DNA seropositive ducks, and only in 3 of 68 (4%) seronegative ducks. The three ducks were all from a heavily infected flock in southern China. Serial analyses of viral DNA by Southern blot and spot hybridizations in experimentally infected Japanese ducklings revealed a dissociation or a time gap between the amount of viral DNA in serum and the emergence of orcein positive hepatocytes. Orcein‐positive hepatocytes were generally associated with prolonged presence of viral infection for at least 4 to 6 months. These findings support the clinical hypothesis that the presence of orcein‐positive hepatocytes indicates persistent rather than acute infection. Since orcein‐positive hepatocytes have been seen in infection with hepatitis B, woodchuck hepatitis, ground squirrel and duck hepatitis B viruses, accumulation of orcein‐positive material in liver cells may be one of the common properties these viruses share. This stain may be utilized for screening new hepatitis B virus‐like viruses.
ISSN:0270-9139
1527-3350
DOI:10.1002/hep.1840040605