Properties of Thymidine Kinase Partially Purified from Human Fetal and Adult Tissue

Properties of thymidine kinase, partially purified from human fetal liver, have been compared with properties of human adult thymidine kinase. Fetal thymidine kinase differs from the adult form in electrophoretic properties, pH optima, heat stability, inhibition by dCTP, and ability to utilize nucle...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1972-03, Vol.247 (6), p.1930-1935
Main Authors: Taylor, A T, Stafford, M A, Jones, O W
Format: Article
Language:English
Subjects:
Adenosine Triphosphate
Age Factors
Carcinoma - enzymology
Cell Line
Cell Nucleus - enzymology
Cytoplasm - enzymology
Cytosine Nucleotides
Drug Stability
Electrophoresis
Female
HeLa Cells - enzymology
Hot Temperature
Humans
Hydrogen-Ion Concentration
Liver - cytology
Liver - embryology
Liver - enzymology
Mitochondria, Liver - enzymology
Mouth Neoplasms - enzymology
Nucleotides
Pregnancy
Spleen - enzymology
Subcellular Fractions
Thymidine Kinase - antagonists & inhibitors
Thymidine Kinase - isolation & purification
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Properties of thymidine kinase, partially purified from human fetal liver, have been compared with properties of human adult thymidine kinase. Fetal thymidine kinase differs from the adult form in electrophoretic properties, pH optima, heat stability, inhibition by dCTP, and ability to utilize nucleotides other than ATP as phosphate donors. We observe that human fetal thymidine kinase resembles thymidine kinase present in at least two permanent human tumor cell lines and also has properties similar to the enzyme found by other workers in a variety of animal and human tumor cells.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)45559-2