Loading…
Properties of Thymidine Kinase Partially Purified from Human Fetal and Adult Tissue
Properties of thymidine kinase, partially purified from human fetal liver, have been compared with properties of human adult thymidine kinase. Fetal thymidine kinase differs from the adult form in electrophoretic properties, pH optima, heat stability, inhibition by dCTP, and ability to utilize nucle...
Saved in:
| Published in: | The Journal of biological chemistry 1972-03, Vol.247 (6), p.1930-1935 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c378t-f49396f5ee3914d41c005def795ec86fa9c2dde0eb06613d536a4c5c5e95501a3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c378t-f49396f5ee3914d41c005def795ec86fa9c2dde0eb06613d536a4c5c5e95501a3 |
| container_end_page | 1935 |
| container_issue | 6 |
| container_start_page | 1930 |
| container_title | The Journal of biological chemistry |
| container_volume | 247 |
| creator | Taylor, A T Stafford, M A Jones, O W |
| description | Properties of thymidine kinase, partially purified from human fetal liver, have been compared with properties of human adult
thymidine kinase. Fetal thymidine kinase differs from the adult form in electrophoretic properties, pH optima, heat stability,
inhibition by dCTP, and ability to utilize nucleotides other than ATP as phosphate donors.
We observe that human fetal thymidine kinase resembles thymidine kinase present in at least two permanent human tumor cell
lines and also has properties similar to the enzyme found by other workers in a variety of animal and human tumor cells. |
| doi_str_mv | 10.1016/S0021-9258(19)45559-2 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_81398696</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>81398696</sourcerecordid><originalsourceid>FETCH-LOGICAL-c378t-f49396f5ee3914d41c005def795ec86fa9c2dde0eb06613d536a4c5c5e95501a3</originalsourceid><addsrcrecordid>eNo9kEtLAzEQx4MotVY_QiF4ED2sJptNujlKsVYsWGgFbyFNJjayj5rsIv32bh90LnP4P2b4ITSk5JESKp4WhKQ0kSnP76l8yDjnMknPUJ-SnCWM069z1D9ZLtFVjD-km0zSHupxQtORyPtoMQ_1BkLjIeLa4eV6W3rrK8DvvtIR8Fx3mi6KLZ63wTsPFrtQl3jalrrCE2h0gXVl8bNtiwYvfYwtXKMLp4sIN8c9QJ-Tl-V4msw-Xt_Gz7PEsFHeJC6TTArHAZikmc2oIYRbcCPJweTCaWlSa4HAighBmeVM6Mxww0Hy7n_NBuju0LsJ9W8LsVGljwaKQldQt1HllMlcSNEZ-cFoQh1jAKc2wZc6bBUlagdT7WGqHSlFpdrDVGmXGx4PtKsS7Cl1pNfptwd97b_Xfz6AWvnarKFUaTZSoqtihP0DxcV7OQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>81398696</pqid></control><display><type>article</type><title>Properties of Thymidine Kinase Partially Purified from Human Fetal and Adult Tissue</title><source>ScienceDirect (Online service)</source><creator>Taylor, A T ; Stafford, M A ; Jones, O W</creator><creatorcontrib>Taylor, A T ; Stafford, M A ; Jones, O W</creatorcontrib><description>Properties of thymidine kinase, partially purified from human fetal liver, have been compared with properties of human adult
thymidine kinase. Fetal thymidine kinase differs from the adult form in electrophoretic properties, pH optima, heat stability,
inhibition by dCTP, and ability to utilize nucleotides other than ATP as phosphate donors.
We observe that human fetal thymidine kinase resembles thymidine kinase present in at least two permanent human tumor cell
lines and also has properties similar to the enzyme found by other workers in a variety of animal and human tumor cells.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(19)45559-2</identifier><identifier>PMID: 5012768</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Adenosine Triphosphate ; Age Factors ; Carcinoma - enzymology ; Cell Line ; Cell Nucleus - enzymology ; Cytoplasm - enzymology ; Cytosine Nucleotides ; Drug Stability ; Electrophoresis ; Female ; HeLa Cells - enzymology ; Hot Temperature ; Humans ; Hydrogen-Ion Concentration ; Liver - cytology ; Liver - embryology ; Liver - enzymology ; Mitochondria, Liver - enzymology ; Mouth Neoplasms - enzymology ; Nucleotides ; Pregnancy ; Spleen - enzymology ; Subcellular Fractions ; Thymidine Kinase - antagonists & inhibitors ; Thymidine Kinase - isolation & purification ; Time Factors</subject><ispartof>The Journal of biological chemistry, 1972-03, Vol.247 (6), p.1930-1935</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-f49396f5ee3914d41c005def795ec86fa9c2dde0eb06613d536a4c5c5e95501a3</citedby><cites>FETCH-LOGICAL-c378t-f49396f5ee3914d41c005def795ec86fa9c2dde0eb06613d536a4c5c5e95501a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5012768$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Taylor, A T</creatorcontrib><creatorcontrib>Stafford, M A</creatorcontrib><creatorcontrib>Jones, O W</creatorcontrib><title>Properties of Thymidine Kinase Partially Purified from Human Fetal and Adult Tissue</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Properties of thymidine kinase, partially purified from human fetal liver, have been compared with properties of human adult
thymidine kinase. Fetal thymidine kinase differs from the adult form in electrophoretic properties, pH optima, heat stability,
inhibition by dCTP, and ability to utilize nucleotides other than ATP as phosphate donors.
We observe that human fetal thymidine kinase resembles thymidine kinase present in at least two permanent human tumor cell
lines and also has properties similar to the enzyme found by other workers in a variety of animal and human tumor cells.</description><subject>Adenosine Triphosphate</subject><subject>Age Factors</subject><subject>Carcinoma - enzymology</subject><subject>Cell Line</subject><subject>Cell Nucleus - enzymology</subject><subject>Cytoplasm - enzymology</subject><subject>Cytosine Nucleotides</subject><subject>Drug Stability</subject><subject>Electrophoresis</subject><subject>Female</subject><subject>HeLa Cells - enzymology</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Liver - cytology</subject><subject>Liver - embryology</subject><subject>Liver - enzymology</subject><subject>Mitochondria, Liver - enzymology</subject><subject>Mouth Neoplasms - enzymology</subject><subject>Nucleotides</subject><subject>Pregnancy</subject><subject>Spleen - enzymology</subject><subject>Subcellular Fractions</subject><subject>Thymidine Kinase - antagonists & inhibitors</subject><subject>Thymidine Kinase - isolation & purification</subject><subject>Time Factors</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><recordid>eNo9kEtLAzEQx4MotVY_QiF4ED2sJptNujlKsVYsWGgFbyFNJjayj5rsIv32bh90LnP4P2b4ITSk5JESKp4WhKQ0kSnP76l8yDjnMknPUJ-SnCWM069z1D9ZLtFVjD-km0zSHupxQtORyPtoMQ_1BkLjIeLa4eV6W3rrK8DvvtIR8Fx3mi6KLZ63wTsPFrtQl3jalrrCE2h0gXVl8bNtiwYvfYwtXKMLp4sIN8c9QJ-Tl-V4msw-Xt_Gz7PEsFHeJC6TTArHAZikmc2oIYRbcCPJweTCaWlSa4HAighBmeVM6Mxww0Hy7n_NBuju0LsJ9W8LsVGljwaKQldQt1HllMlcSNEZ-cFoQh1jAKc2wZc6bBUlagdT7WGqHSlFpdrDVGmXGx4PtKsS7Cl1pNfptwd97b_Xfz6AWvnarKFUaTZSoqtihP0DxcV7OQ</recordid><startdate>19720325</startdate><enddate>19720325</enddate><creator>Taylor, A T</creator><creator>Stafford, M A</creator><creator>Jones, O W</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19720325</creationdate><title>Properties of Thymidine Kinase Partially Purified from Human Fetal and Adult Tissue</title><author>Taylor, A T ; Stafford, M A ; Jones, O W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-f49396f5ee3914d41c005def795ec86fa9c2dde0eb06613d536a4c5c5e95501a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>Adenosine Triphosphate</topic><topic>Age Factors</topic><topic>Carcinoma - enzymology</topic><topic>Cell Line</topic><topic>Cell Nucleus - enzymology</topic><topic>Cytoplasm - enzymology</topic><topic>Cytosine Nucleotides</topic><topic>Drug Stability</topic><topic>Electrophoresis</topic><topic>Female</topic><topic>HeLa Cells - enzymology</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Liver - cytology</topic><topic>Liver - embryology</topic><topic>Liver - enzymology</topic><topic>Mitochondria, Liver - enzymology</topic><topic>Mouth Neoplasms - enzymology</topic><topic>Nucleotides</topic><topic>Pregnancy</topic><topic>Spleen - enzymology</topic><topic>Subcellular Fractions</topic><topic>Thymidine Kinase - antagonists & inhibitors</topic><topic>Thymidine Kinase - isolation & purification</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Taylor, A T</creatorcontrib><creatorcontrib>Stafford, M A</creatorcontrib><creatorcontrib>Jones, O W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Taylor, A T</au><au>Stafford, M A</au><au>Jones, O W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Properties of Thymidine Kinase Partially Purified from Human Fetal and Adult Tissue</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1972-03-25</date><risdate>1972</risdate><volume>247</volume><issue>6</issue><spage>1930</spage><epage>1935</epage><pages>1930-1935</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Properties of thymidine kinase, partially purified from human fetal liver, have been compared with properties of human adult
thymidine kinase. Fetal thymidine kinase differs from the adult form in electrophoretic properties, pH optima, heat stability,
inhibition by dCTP, and ability to utilize nucleotides other than ATP as phosphate donors.
We observe that human fetal thymidine kinase resembles thymidine kinase present in at least two permanent human tumor cell
lines and also has properties similar to the enzyme found by other workers in a variety of animal and human tumor cells.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>5012768</pmid><doi>10.1016/S0021-9258(19)45559-2</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1972-03, Vol.247 (6), p.1930-1935 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81398696 |
| source | ScienceDirect (Online service) |
| subjects | Adenosine Triphosphate Age Factors Carcinoma - enzymology Cell Line Cell Nucleus - enzymology Cytoplasm - enzymology Cytosine Nucleotides Drug Stability Electrophoresis Female HeLa Cells - enzymology Hot Temperature Humans Hydrogen-Ion Concentration Liver - cytology Liver - embryology Liver - enzymology Mitochondria, Liver - enzymology Mouth Neoplasms - enzymology Nucleotides Pregnancy Spleen - enzymology Subcellular Fractions Thymidine Kinase - antagonists & inhibitors Thymidine Kinase - isolation & purification Time Factors |
| title | Properties of Thymidine Kinase Partially Purified from Human Fetal and Adult Tissue |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T06%3A36%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Properties%20of%20Thymidine%20Kinase%20Partially%20Purified%20from%20Human%20Fetal%20and%20Adult%20Tissue&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=Taylor,%20A%20T&rft.date=1972-03-25&rft.volume=247&rft.issue=6&rft.spage=1930&rft.epage=1935&rft.pages=1930-1935&rft.issn=0021-9258&rft.eissn=1083-351X&rft_id=info:doi/10.1016/S0021-9258(19)45559-2&rft_dat=%3Cproquest_cross%3E81398696%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c378t-f49396f5ee3914d41c005def795ec86fa9c2dde0eb06613d536a4c5c5e95501a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=81398696&rft_id=info:pmid/5012768&rfr_iscdi=true |