The Effect of Erythropoietin on Synchronized Rabbit Hemopoietic Tissue in Culture

Synchronized erythroid cell cohorts were cultured for up to 6 days with and without added erythropoietin. The presence of erythropoietin in the medium was found indispensable for the synchronized differentiation and maturation of those cell populations. The appearance, during maturation in vitro, of...

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Bibliographic Details
Published in:Blood 1972-06, Vol.39 (6), p.814-825
Main Authors: Izak, G., Karsai, A.
Format: Article
Language:English
Subjects:
Amino Acids - metabolism
Animals
Blood Proteins - biosynthesis
Carbon Isotopes
Cell Differentiation - drug effects
Cell Fractionation
Cells, Cultured
DNA - biosynthesis
Electrophoresis, Disc
Erythrocytes - drug effects
Erythropoietin - pharmacology
Hematopoietic Stem Cells - drug effects
Heme - biosynthesis
Rabbits
RNA - biosynthesis
Thymidine - metabolism
Tritium
Uridine - metabolism
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Summary:Synchronized erythroid cell cohorts were cultured for up to 6 days with and without added erythropoietin. The presence of erythropoietin in the medium was found indispensable for the synchronized differentiation and maturation of those cell populations. The appearance, during maturation in vitro, of a soluble cell component that is capable of inducing hemoglobin producation by polysomes isolated from undifferentiated blast cells is described. The same polysomes with their isologous soluble cell component were unable to produce this protein. These results confirm earlier observations made on synchronized rabbit cell populations in vivo.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V39.6.814.814