Versatile System for Partition Chromatography of Corticosteroids and Prediction of Their Elution Curves

• Published in: Journal of pharmaceutical sciences 1972-05, Vol.61 (5), p.689-694
• Main Authors: Weber, D.J., Ennals, T.R., Mitchner, H.
• Format: Article
• Language: English
• Subjects: 17 corticosteroids-versatile solvent system; Acetates; Adrenal Cortex Hormones; Aldehydes; Betamethasone; Chemical Phenomena; Chemistry; Chromatography; Chromatography, partition—versatile solvent system for corticosteroids, prediction of elution curves; Corticosteroids-separation by partition chromatography; Corticosteroids—separation by partition chromatography, versatile solvent system, prediction of elution curves; Cortisone; Flumethasone; Fluocinolone Acetonide; Hydrocortisone; Kinetics; Methods; Paramethasone; Partition chromatography; Partition chromatography, 17 corticosteroids—versatile solvent system, prediction of elution curves from partition coefficients, substituent effect; partition-versatile solvent system for corticosteroids; prediction of elution curves; prediction of elution curves from partition coefficients; Prednisolone; Prednisone; Progesterone; Solvents; substituent effect; Triamcinolone Acetonide; versatile solvent system
• ISSN: 0022-3549; 1520-6017
• DOI: 10.1002/jps.2600610504

The versatile solvent system of n-hexane-chloroform-dioxane-water in the ratio 90:10:40:5 was used for the partition chromatographic isolation of corticosteroids. The relative amounts of the components may be adjusted if necessary to give a convenient partition coefficient on a diatomaceous earth column. Data are presented for 17 steroids, showing the close agreement between partition coefficients predicted from chromatographic elution curve data and those calculated from solvent extraction data. A procedure for the experimental determination of column parameters is given, and the necessary equations are outlined. The prediction of peak elution volumes and solute bandwidths as a function of partition coefficients from the derived equations is demonstrated, and the quantitative effect of varying the stationary phase volume and column length is calculated. The effect of steroid side-chain structure and fluoride substitution on the partition coefficient was studied. The effect of the substituents can be estimated from their expected polarity.