Inhibition of Aggregation and Differentiation of Dictyostelium discoideum by Antibodies against Adenosine 3′:5′-cyclic Monophosphate Diesterase

A specific antibody directed against adenosine 3′:5′-cyclic monophosphate diesterase (c-AMP diesterase; EC 3.1.4.c), which is secreted by the amoeboid slime mold, Dictyostelium discoideum, was prepared in rabbits. Purified gammaglobulin fractions that contain the antibodies inhibited the activity of...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1972-05, Vol.69 (5), p.1128-1130
Main Authors: Goidl, Edmond A., Chassy, Bruce M., Love, Leslie L., Krichevsky, Micah I.
Format: Article
Language:English
Subjects:
Aggregation
Animals
Antibodies
Antibody Specificity
Antigens
Antiserum
Biological Sciences: Immunology
Cell aggregates
Cell Aggregation
Cell Differentiation
Chemotaxis
Cyclic AMP
Enzyme activity
Enzymes
gamma-Globulins - pharmacology
Globulins
Immune Sera
Immunization
Myxomycetes - enzymology
Myxomycetes - immunology
Phosphates
Phosphoric Monoester Hydrolases
Rabbits
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A specific antibody directed against adenosine 3′:5′-cyclic monophosphate diesterase (c-AMP diesterase; EC 3.1.4.c), which is secreted by the amoeboid slime mold, Dictyostelium discoideum, was prepared in rabbits. Purified gammaglobulin fractions that contain the antibodies inhibited the activity of cAMP diesterase preparations in vitro and interfered with aggregation and development in vivo. While cells that were treated with antibody were unable to aggregate because of the inability to destroy cAMP, they aggregated normally when washed free of antibody.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.69.5.1128