Opposite effects of Trichostatin A on activation of mast cells by different stimulants

• Published in: FEBS letters 2010-06, Vol.584 (11), p.2315-2320
• Main Authors: Wang, Qing-hui, Nishiyama, Chiharu, Nakano, Nobuhiro, Kanada, Shunsuke, Hara, Mutsuko, Kitamura, Nao, Shimokawa, Naomi, Lu, Chang-long, Ogawa, Hideoki, Okumura, Ko
• Format: Article
• Language: English
• Subjects: BMMC; bone marrow-derived mast cell; ChIP; chromatin immunoprecipitation; cycle threshold; FcεRI; HAT; HDAC; HDACi; histone acetyltransferase; histone deacetylase; histone deacetylase inhibitors; Hydroxamic Acids - pharmacology; Interleukin-6 - genetics; Interleukin-6 - metabolism; Lipopolysaccharides - pharmacology; Mast cell; Mast Cells - drug effects; Mast Cells - metabolism; NF-kappa B - genetics; NF-kappa B - metabolism; Pharmacology; propidium iodide; SAHA; Signal Transduction - drug effects; SLE; suberoylanilide hydroxamic acid; systemic lupus erythematosus; Transcription factor; Transcription Factors - genetics; Transcription Factors - metabolism; Trichostatin A; TSA
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2010.03.047

Mast cells (MCs) are activated upon stimulation via TLRs or FcεRI, contributing to immune protection and/or leading to allergic diseases. In the present study, the effects of Trichostatin A (TSA) on the activation of MCs were analyzed with bone marrow-derived (BM) MCs. TSA increased the transcription and protein secretion of IL-6 in case of LPS-stimulation, in contrast to the suppressive effect on IgE-mediated activation of BMMCs. Chromatin immunoprecipitation assay showed IgE-mediated signaling-specific suppression of transcription factors recruitment to the IL-6 promoter. TSA-treatment inhibited nuclear translocation of NF-κB following IgE-mediated, but not LPS-induced activation in MCs.