Application of NMR Spectroscopy and Imaging in Heterogeneous Biocatalysis

Heterogeneously catalyzed enzymatic glucose isomerization was considered as a model process to extend the application of nuclear magnetic resonance (NMR) and magnetic resonance imaging techniques to the studies of biocatalytic processes and heterogeneous biocatalysts. It has been demonstrated that t...

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Bibliographic Details
Published in:Applied magnetic resonance 2010, Vol.37 (1-4), p.483-495
Main Authors: Koptyug, Irina I., Lysova, Anna A., Kovalenko, Galina A., Perminova, Larisa V., Koptyug, Igor V.
Format: Article
Language:English
Subjects:
Aqueous solutions
Atoms and Molecules in Strong Fields
Biocatalysts
Biofilms
Enzymes
Experiments
Fructose
Glucose
Imaging techniques
Isomerization
Laser Matter Interaction
Magnetic resonance imaging
Microorganisms
NMR
NMR spectroscopy
Nuclear magnetic resonance
Nuclear reactors
Organic Chemistry
Overhauser effect
Packed beds
Physical Chemistry
Physics
Physics and Astronomy
Signal to noise ratio
Solid State Physics
Spatial distribution
Spectra
Spectroscopy/Spectrometry
Spectrum analysis
Spin decoupling
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Summary:Heterogeneously catalyzed enzymatic glucose isomerization was considered as a model process to extend the application of nuclear magnetic resonance (NMR) and magnetic resonance imaging techniques to the studies of biocatalytic processes and heterogeneous biocatalysts. It has been demonstrated that the T 2 times of glucose are different for its aqueous solution in the pores of an unmodified porous support and in a heterogeneous biocatalyst, comprising bacterial cells immobilized on the same support. This observation has been used to map the spatial distribution of the active component within a packed bed of biocatalyst in a model reactor. 13 C NMR spectroscopy was applied to follow the progress of glucose isomerization catalyzed by the heterogeneous biocatalyst in a batch reactor. The utilization of proton spin decoupling and nuclear Overhauser effect was shown to be necessary to obtain high signal-to-noise ratio in the natural abundance 13 C NMR spectra of a glucose–fructose syrup present in the packed bed of biocatalyst. The spectra thus obtained were suitable for the quantification of the glucose-to-fructose ratio achieved in the biocatalytic reaction.
ISSN:0937-9347
1613-7507
DOI:10.1007/s00723-009-0074-7